Institute of Grain and Oil Quality Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, PR China.
Institute of Grain and Oil Quality Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, PR China; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, PR China.
Food Chem. 2023 Oct 1;422:136226. doi: 10.1016/j.foodchem.2023.136226. Epub 2023 Apr 26.
In this study, based on the high-throughput automatic sample pretreatment with immunoaffinity magnetic beads with oriented immobilized antibodies, grain and feed fumonisin (FB) content was detected using pre-column automatic derivatization of high-performance liquid chromatography (HPLC). The FB capacity of well-oriented antibody immunoaffinity magnetic beads was 1.5-1.8 times that of magnetic beads with randomly fixed antibody. This pre-column automatic derivatization method using an autosampler can reduce error from manual injection and improve detection efficiency. The spiked recoveries for three different concentrations in maize, husked rice, and pig feed under optimized conditions were 84.6-104.0% (RSD < 9.3%). Our novel method was also applied to the analysis of FBs in 63 maize samples collected from the main maize-production regions in China. The results showed that as latitude increased, the contamination level of FBs tended to decrease. High temperature and high humidity are also more favorable for FB growth.
本研究采用高通量自动免疫亲和磁珠预处理方法，通过定向固定抗体，利用高效液相色谱柱前自动衍生化技术检测粮食和饲料中的伏马菌素（FB）含量。定向抗体免疫亲和磁珠的 FB 容量是随机固定抗体磁珠的 1.5-1.8 倍。该自动进样器柱前自动衍生化方法可减少手动进样的误差，提高检测效率。在优化条件下，玉米、糙米和猪饲料中三种不同浓度的添加回收率为 84.6-104.0%（RSD<9.3%）。我们的新方法还应用于分析来自中国主要玉米产区的 63 个玉米样品中的 FB。结果表明，随着纬度的增加，FB 的污染水平呈下降趋势。高温高湿也更有利于 FB 的生长。
