University of Lisbon, Faculty of Sciences, Department of Chemistry and Biochemistry and BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, C8, 1749-016 Lisboa, Portugal.
Instituto de Tecnologia Química e Biológica - António Xavier, Universidade Nova de Lisboa, Av. da República EAN, 2780-157 Oeiras, Portugal.
Biochim Biophys Acta Bioenerg. 2023 Aug 1;1864(3):148983. doi: 10.1016/j.bbabio.2023.148983. Epub 2023 Apr 29.
Rhodothermus marinus is a thermohalophilic organism that has optimized its microaerobic metabolism at 65 °C. We have been exploring its respiratory chain and observed the existence of a quinone:cytochrome c oxidoreductase complex, named Alternative Complex III, structurally different from the bc complex. In the present work, we took profit from nanodiscs and liposomes technology to investigate ACIII activity in membrane-mimicking systems. In addition, we studied the interaction of ACIII with menaquinone, its potential electron acceptors (HiPIP and cytochrome c) and the caa oxygen reductase.
嗜热盐菌是一种嗜热嗜盐生物，已在 65°C 下优化了其微好氧代谢。我们一直在探索其呼吸链，并观察到存在一种醌：细胞色素 c 氧化还原酶复合物，称为替代复合物 III，其结构与 bc 复合物不同。在本工作中，我们利用纳米盘和脂质体技术在模拟膜系统中研究 ACIII 的活性。此外，我们还研究了 ACIII 与menaquinone、其潜在电子受体（HiPIP 和细胞色素 c）以及 caa 氧还原酶的相互作用。
