Wang Kan, Liu Yuandong, Liu Run, Belqadi Wissal, Zeng Weimin, Yu Runlan, Wu Xueling
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
Key Laboratory of Biohydrometallurgy of Ministry of Education, Changsha 410083, China.
Life (Basel). 2025 May 15;15(5):792. doi: 10.3390/life15050792.
The genus has been widely used in bioleaching, and novel strains in this genus, such as , have also been confirmed to possess bioleaching capabilities. In this study, an strain, QBS3, was isolated from zinc-rich sulfide mine drainage using the gradient dilution method. QBS3 is a Gram-negative, 1.3 µm rod-shaped bacterium with small red colonies. It showed a high iron oxidation efficiency of 0.361 g/(L·h) and a sulfur oxidation efficiency of 0.206 g/(L·d). QBS3 has sphalerite bioleaching ability; using QBS3 for pure sphalerite bioleaching, 18.8% of zinc was extracted in 14 days at 1% pulp density. Whole genome sequencing was performed on QBS3. Functional prediction showed that 9.13% of the genes were involved in replication, recombination, and repair. Bioleaching-related genes were analyzed, including iron and sulfur oxidation genes, and carbon and nitrogen fixation genes. For iron oxidation, the Cyc2→RusA pathway and Iro→RusB pathway were found in QBS3. In terms of sulfur oxidation, QBS3 has an incomplete SOX system and lacks the SDO gene, but Rho and Trx may complement the SOX system, enabling QBS3 to oxidize sulfur. QBS3 has multiple sets of carbon fixation genes, and nitrogen fixation genes were also identified. A hypothetical sphalerite bioleaching model is proposed; this study provides a theoretical basis for the zinc sulfide ore bioleaching industry.
该属已广泛应用于生物浸出领域，该属中的新菌株，如 ，也已被证实具有生物浸出能力。在本研究中，使用梯度稀释法从富含锌的硫化矿排水中分离出一株 菌株QBS3。QBS3是革兰氏阴性菌，杆状，长1.3 µm，菌落呈小红色。它显示出高铁氧化效率，为0.361 g/(L·h)，硫氧化效率为0.206 g/(L·d)。QBS3具有闪锌矿生物浸出能力；在1%矿浆密度下，使用QBS3对纯闪锌矿进行生物浸出，14天内可提取18.8%的锌。对QBS3进行了全基因组测序。功能预测表明，9.13%的基因参与复制、重组和修复。分析了与生物浸出相关的基因，包括铁和硫氧化基因以及碳和氮固定基因。对于铁氧化，在QBS3中发现了Cyc2→RusA途径和Iro→RusB途径。在硫氧化方面，QBS3具有不完整的SOX系统且缺乏SDO基因，但Rho和Trx可能补充SOX系统，使QBS3能够氧化硫。QBS3有多套碳固定基因，并且也鉴定出了氮固定基因。提出了一个假设的闪锌矿生物浸出模型；本研究为硫化锌矿石生物浸出工业提供了理论依据。
