L. 的固体草药提取物可改善慢性酒精中毒大鼠心肌的形态功能状况。

Solid herbal extract of L. improves morphofunctional condition of rats' myocardium in chronic alcohol intoxication.

作者信息

Prokofiev Igor I, Kustova Margarita V, Nesterova Alla A, Perfilova Valentina N, Khusainova Gulnara H, Borodkina Ludmila E, Tivon Yakov V, Tyurenkov Ivan N, Kataev Valeri A, Latypova Gusel M

机构信息

Department of Forensic Medicine, Volgograd State Medical University, 400049, Volgograd, Rokossovsky st.,1g, Russian Federation.

Department for Basic and Clinical Biochemistry, Volgograd State Medical University, 400131, Volgograd, Pavshikh Bortsov Sq.,1, Russian Federation.

出版信息

J Tradit Complement Med. 2023 Feb 22;13(3):306-314. doi: 10.1016/j.jtcme.2023.02.006. eCollection 2023 May.

DOI:10.1016/j.jtcme.2023.02.006

PMID:37128197

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10148137/

Abstract

BACKGROUND AND AIM

Chronic alcohol intoxication (CAI) induces heart damage. One of the promising ways of its treatment involves the administration of herbal medicinal products. The purpose of this study was to explore the effect of solid herbal extract of Primula veris L. (PVSHE) on the morphofunctional changes in rats' myocardium after CAI.

EXPERIMENTAL PROCEDURE

CAI was simulated for 24 weeks. Loading testing was used to assess the functional condition of the heart, the functional assessment of mitochondria was based on the polarographic determination of oxygen consumption rate and determination of the indices of lipid peroxidation and antioxidant enzymes activity. We performed a microscopic examination of the left ventricle following the standard protocol of histological processing and h&e staining.

RESULTS AND CONCLUSION

PVSHE restricts the toxic effects of ethanol on the heart which was indicated by a higher rise in the rates of myocardial contraction (by an average of 3.9 times, P < 0.05) and relaxation (2.6 times under volume load, P < 0.05), LVP (by an average of 1.7 times, P < 0.05) and MISP (by an average of 1.5 times, P < 0.05). PVSHE caused an improvement in the functional state of rats' cardiac mitochondria exposed to CAI, which was demonstrated by on average 1.3-1.4 times (P < 0.05) as high RCR as compared to the control group. The histological examination of the myocardium of the animals treated with PVSHE showed the increase in the volume fraction of cardiac myocytes, and a 31.2% (P < 0.05) decline in the interstitial volume. Therefore, PVSHE has a protective effect on the heart after CAI.

摘要

背景与目的

慢性酒精中毒（CAI）会导致心脏损伤。其一种有前景的治疗方法是使用草药产品。本研究的目的是探讨报春花固体草药提取物（PVSHE）对CAI后大鼠心肌形态功能变化的影响。

实验过程

模拟CAI 24周。采用负荷试验评估心脏功能状态，基于极谱法测定氧消耗率以及测定脂质过氧化指标和抗氧化酶活性对线粒体进行功能评估。按照组织学处理和苏木精-伊红染色的标准方案对左心室进行显微镜检查。

结果与结论

PVSHE可限制乙醇对心脏的毒性作用，这表现为心肌收缩率（平均升高3.9倍，P<0.05）和舒张率（容量负荷下升高2.6倍，P<0.05）、左心室压力（平均升高1.7倍，P<0.05）和心肌等容收缩期压力（平均升高1.5倍，P<0.05）有更高幅度的升高。PVSHE使暴露于CAI的大鼠心脏线粒体功能状态得到改善，与对照组相比，呼吸控制率平均高1.3 - 1.4倍（P<0.05）。对用PVSHE治疗的动物心肌进行组织学检查显示心肌细胞体积分数增加，间质体积下降31.2%（P<0.05）。因此，PVSHE对CAI后的心脏具有保护作用。