Ishaq Suzanne L, Turner Sarah M, Lee Grace, Tudor M Scarlett, MacRae Jean D, Hamlin Heather, Bouchard Deborah
School of Food and Agriculture, University of Maine, Orono, Maine 04469, USA.
Aquaculture Research Institute, University of Maine, Orono, Maine 04469, USA.
iScience. 2023 Apr 8;26(5):106606. doi: 10.1016/j.isci.2023.106606. eCollection 2023 May 19.
The American lobster, , is an economically valuable and ecologically important crustacean along the North Atlantic coast of North America. Populations in southern locations have declined in recent decades due to increasing ocean temperatures and disease, and these circumstances are progressing northward. We monitored 57 adult female lobsters, healthy and shell diseased, under three seasonal temperature cycles for a year, to track shell bacterial communities using culturing and 16S rRNA gene sequencing, progression of epizootic shell disease using visual assessment, and antimicrobial activity of hemolymph. The richness of bacterial taxa present, evenness of abundance, and community similarity between lobsters was affected by water temperature at the time of sampling, water temperature over time based on seasonal temperature regimes, shell disease severity, and molt stage. Several bacteria were prevalent on healthy lobster shells but missing or less abundant on diseased shells, although some bacteria were found on all shells regardless of health status.
美洲龙虾（Homarus americanus）是北美北大西洋沿岸一种具有经济价值且对生态重要的甲壳类动物。近几十年来，由于海洋温度上升和疾病，南部地区的龙虾种群数量有所下降，而且这些情况正在向北蔓延。我们对57只成年雌性龙虾（健康的和患有壳病的）进行了为期一年的三个季节性温度循环监测，通过培养和16S rRNA基因测序来追踪壳细菌群落，通过视觉评估来观察 epizootic 壳病的进展情况，并检测血淋巴的抗菌活性。采样时的水温、基于季节性温度模式的随时间变化的水温、壳病严重程度和蜕壳阶段会影响龙虾身上存在的细菌分类群的丰富度、丰度均匀度以及龙虾之间的群落相似性。尽管无论健康状况如何，在所有壳上都发现了一些细菌，但几种细菌在健康龙虾壳上普遍存在，而在患病壳上缺失或数量较少。
