Pediatric Immunology and Rheumatology, Division of Pediatrics, University Medical Center Utrecht, Utrecht, The Netherlands.
Faculty of Medicine, Utrecht University, Utrecht, The Netherlands.
Int J Lab Hematol. 2023 Oct;45(5):659-667. doi: 10.1111/ijlh.14080. Epub 2023 May 2.
This study assessed the comparability of complete blood count (CBC) parameters between capillary and venous samples, and extended previous research by examining the influence of different storage temperatures on CBC stability up to 7 days after sample collection.
Venous and capillary blood samples were collected from 93 adult patients. Hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean platelet volume (MPV), leukocytes, lymphocytes, basophils, eosinophils, erythrocytes, red cell distribution width (RDW), immature granulocytes (IG), immature reticulocyte fraction (IRF), monocytes, neutrophils, platelets, and reticulocytes were measured. Deming regression and mean relative differences between venous and capillary measurements were contrasted with desirable total allowable error (TEa). Stability was assessed in 20-27 venous blood samples stored at 4, 21-22, or 30°C, and analyzed at 0, 24, 48, 72, 96, 120, 144, and 168 h. Mean relative change with respect to baseline measurements was compared to the desirable TEa to determine acceptable stability.
Deming regression demonstrated strong linear correlations and acceptable variation between venous and capillary measurements. Erythrocytes, Hb, Ht, MCH, MCV, RDW, reticulocytes, and platelets showed acceptable stability for at least 96 h at 4°C. Mean relative change exceeded desirable TEa after 24 h at 30°C for all parameters, except erythrocytes, Hb, leukocytes, and MCH.
Clinical laboratory specialists and clinicians should be aware of potential differences between venous and capillary measurements, and the influence of storage conditions. Clinical validity of delayed CBC analysis depends on the clinical situation and required precision of the result.
本研究评估了毛细血管和静脉样本之间全血细胞计数 (CBC) 参数的可比性，并通过检查不同储存温度对 CBC 稳定性的影响，扩展了之前的研究，即在样本采集后 7 天内检测 CBC 稳定性。
从 93 名成年患者中采集静脉和毛细血管血样。测量血红蛋白 (Hb)、红细胞压积 (Ht)、平均红细胞体积 (MCV)、平均红细胞血红蛋白 (MCH)、平均红细胞血红蛋白浓度 (MCHC)、平均血小板体积 (MPV)、白细胞、淋巴细胞、嗜碱性粒细胞、嗜酸性粒细胞、红细胞、红细胞分布宽度 (RDW)、未成熟粒细胞 (IG)、未成熟网织红细胞分数 (IRF)、单核细胞、中性粒细胞、血小板和网织红细胞。对比静脉和毛细血管测量的 Deming 回归和平均相对差异与理想的总允许误差 (TEa)。将 20-27 份静脉血样本储存在 4°C、21-22°C 或 30°C 下，在 0、24、48、72、96、120、144 和 168 小时时进行分析，并评估稳定性。与基线测量值相比，相对平均变化与理想 TEa 进行比较，以确定可接受的稳定性。
Deming 回归显示静脉和毛细血管测量之间具有很强的线性相关性和可接受的差异。红细胞、Hb、Ht、MCH、MCV、RDW、网织红细胞和血小板在 4°C 下至少 96 小时内具有可接受的稳定性。除红细胞、Hb、白细胞和 MCH 外，所有参数在 30°C 下 24 小时后相对平均变化均超过理想 TEa。
临床实验室专家和临床医生应该意识到静脉和毛细血管测量之间的潜在差异，以及储存条件的影响。延迟 CBC 分析的临床有效性取决于临床情况和结果的所需精度。
