Department of Chemistry, University of California, Davis, CA, 95616, USA.
Biomol NMR Assign. 2023 Jun;17(1):115-119. doi: 10.1007/s12104-023-10129-3. Epub 2023 May 2.
Retinal membrane guanylyl cyclases (RetGCs) in vertebrate rod and cone photoreceptors are activated by a family of neuronal Ca sensor proteins called guanylyl cyclase activating proteins (GCAP1-7). GCAP5 from zebrafish photoreceptors binds to RetGC and confers Ca/Fe-dependent regulation of RetGC enzymatic activity that promotes the recovery phase of visual phototransduction. We report NMR chemical shift assignments of GCAP5 with a R22A mutation (called GCAP5) that abolishes protein dimerization and activates RetGC with 3-fold higher activity than that of wild type GCAP5 (BMRB No. 51,783).
脊椎动物视杆和视锥感光细胞中的视网膜膜鸟苷酸环化酶（RetGCs）被称为鸟苷酸环化酶激活蛋白（GCAP1-7）的一类神经元钙传感器蛋白激活。斑马鱼感光细胞中的 GCAP5 与 RetGC 结合，并赋予其对 Ca/Fe 的依赖性调节，从而促进视觉光传导的恢复阶段。我们报告了 R22A 突变（称为 GCAP5）的 GCAP5 的 NMR 化学位移分配，该突变消除了蛋白质二聚化，并使 RetGC 的活性比野生型 GCAP5 高 3 倍（BMRB No. 51,783）。
