Yang Haoyi, Yang Liang, Du Xiping, He Ning, Jiang Zedong, Zhu Yanbing, Li Lijun, Ni Hui, Li Qingbiao, Li Zhipeng
College of Ocean Food and Biology Engineering, Jimei University, Xiamen, Fujian, China.
Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Technology, Xiamen, Fujian, China.
Yeast. 2023 Jul;40(7):254-264. doi: 10.1002/yea.3854. Epub 2023 May 3.
Astaxanthin is a valuable carotenoid and is used as antioxidant and health care. Phaffia rhodozyma is a potential strain for the biosynthesis of astaxanthin. The unclear metabolic characteristics of P. rhodozyma at different metabolic stages hinder astaxanthin's promotion. This study is conducted to investigate metabolite changes based on quadrupole time-of-flight mass spectrometry metabolomics method. The results showed that the downregulation of purine, pyrimidine, amino acid synthesis, and glycolytic pathways contributed to astaxanthin biosynthesis. Meanwhile, the upregulation of lipid metabolites contributed to astaxanthin accumulation. Therefore, the regulation strategies were proposed based on this. The addition of sodium orthovanadate inhibited the amino acid pathway to increase astaxanthin concentration by 19.2%. And the addition of melatonin promoted lipid metabolism to increase the astaxanthin concentration by 30.3%. It further confirmed that inhibition of amino acid metabolism and promotion of lipid metabolism were beneficial for astaxanthin biosynthesis of P. rhodozyma. It is helpful in understanding metabolic pathways affecting astaxanthin of P. rhodozyma and provides regulatory strategies for metabolism.
虾青素是一种有价值的类胡萝卜素，用作抗氧化剂和保健品。红发夫酵母是虾青素生物合成的潜在菌株。红发夫酵母在不同代谢阶段的代谢特征不明确，阻碍了虾青素的推广。本研究基于四极杆飞行时间质谱代谢组学方法，对代谢物变化进行了研究。结果表明，嘌呤、嘧啶、氨基酸合成和糖酵解途径的下调有助于虾青素的生物合成。同时，脂质代谢物的上调有助于虾青素的积累。因此，基于此提出了调控策略。添加原钒酸钠抑制氨基酸途径，使虾青素浓度提高了19.2%。添加褪黑素促进脂质代谢，使虾青素浓度提高了30.3%。进一步证实，抑制氨基酸代谢和促进脂质代谢有利于红发夫酵母虾青素的生物合成。这有助于了解影响红发夫酵母虾青素的代谢途径，并为代谢提供调控策略。
