The Department of Chemical Engineering and the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Sun Pharmaceutical Industries Australia Pty Ltd., Princes Highway, Port Fairy, Victoria 3281, Australia.
Chem Commun (Camb). 2023 May 18;59(41):6251-6254. doi: 10.1039/d3cc00534h.
A whole cell biotransformation platform converting thebaine to oripavine and codeine to morphine was demonstrated with industrially applicable yields (∼1.2 × 10 g L h or ∼1.2 × 10 g L h), improving >13 400-fold upon morphine production in yeast. Mutations enhanced enzyme performance and the use of a purified substrate with rich raw poppy extract expanded applicability.
利用工业上可行的产率（约 1.2×10 g L h 或约 1.2×10 g L h），展示了一种全细胞生物转化平台，可将蒂巴因转化为异土木防己碱和可待因转化为吗啡，相比于酵母中生产吗啡，提高了>13400 倍。突变增强了酶的性能，而使用富含罂粟原料提取物的纯化底物则扩大了其适用性。
