Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Nigeria, West Africa.
JBRA Assist Reprod. 2023 Sep 12;27(3):386-390. doi: 10.5935/1518-0557.20220069.
Endogenous prostaglandins are involved in hemostasis, renal excretion of electrolytes, and implicated in dysmenorrhea. Piroxicam and Nitroglycerin are common drugs used in treating dysmenorrhea by inhibiting the cyclooxygenase pathway involved in prostaglandin production. However, studies comparing the effects of these drugs on prostaglandin-modulated hemostasis and renal function are lacking.
Fifteen female rats (120-160g) were divided into 3 groups (20 per group), namely Control (distilled water, 0.3 mL), Piroxicam treated (3mg/kg) and Nitroglycerin treated (1 mg/kg). Di-estrous phase was confirmed in animals in each group using the Pipette smear method. Treatment was administered for 4 days covering the estrous cycle. Bleeding and clotting time were assessed and blood concentrations of sodium, potassium, urea and platelet counts were evaluated in all phases. Data were analyzed using one-way ANOVA and Newman-Keuls post-hoc test. Statistical significance was considered at p<0.0.
The nitroglycerin-treated group showed significant increases in blood potassium during di-estrous while the piroxicam-treated group showed significant increases in blood potassium, urea and clotting time with a significant decrease in sodium levels during di-estrous compared to controls. Results obtained in other phases were not significant compared to controls.
The study showed that Nitroglycerin produces minimum alteration of blood and electrolyte indices compared to piroxicam during di-estrous.
内源性前列腺素参与止血、电解质的肾排泄，并与痛经有关。吡罗昔康和硝酸甘油是常用的治疗痛经的药物，通过抑制前列腺素产生过程中的环氧化酶途径。然而，比较这些药物对前列腺素调节的止血和肾功能影响的研究尚缺乏。
将 15 只雌性大鼠（120-160g）分为 3 组（每组 20 只），即对照组（蒸馏水，0.3mL）、吡罗昔康治疗组（3mg/kg）和硝酸甘油治疗组（1mg/kg）。使用吸移管涂片法在每组动物中确认动情周期。治疗期覆盖动情周期，持续 4 天。评估出血和凝血时间，并在所有阶段评估血液中钠、钾、尿素和血小板计数。使用单因素方差分析和 Newman-Keuls 事后检验分析数据。p<0.05 被认为具有统计学意义。
在动情前期，硝酸甘油治疗组的血钾水平显著升高，而吡罗昔康治疗组的血钾、尿素和凝血时间显著升高，与对照组相比，动情前期的钠水平显著降低。与对照组相比，其他阶段的结果没有显著差异。
本研究表明，与吡罗昔康相比，硝酸甘油在动情前期对血液和电解质指标的改变最小。
