Department of Animal Health, NEIKER-Basque Institute for Agricultural Research and Development. Basque Research and Technology Alliance (BRTA), Derio, Spain.
Applied Zoology and Animal Conservation Research Group (ZAP), Department of Biology, University of the Balearic Islands (UIB), Palma de Mallorca, Spain.
Med Vet Entomol. 2023 Sep;37(3):616-629. doi: 10.1111/mve.12661. Epub 2023 May 3.
Mosquitoes (Diptera: Culicidae) are common bloodsucking Diptera frequently found in aquatic environments, which are valuable ecosystems for many animal species, particularly migrating birds. Therefore, interactions between these animal species and mosquitoes may play a critical role in pathogen transmission. During 2018-2019, mosquitoes were collected from two aquatic ecosystems in northern Spain using different methodologies and identified using classical morphology and molecular tools. A total of 1529 males and females of 22 native mosquito species (including eight new records for the region) were trapped using CO -baited Centers for Disease Control and Prevention (CDC) traps and sweep netting. Among the blood-fed female mosquitoes, 11 vertebrate host species-six mammals and five birds-were identified using DNA barcoding. The developmental sites of eight mosquito species were determined across nine microhabitats, and 11 mosquito species were caught landing on humans. The flight period varied among mosquito species, with some peaking in the spring and others in the summer. Our study highlights the advantages of mosquito sampling using various techniques to comprehensively characterise species composition and abundance. Information on the trophic preferences, biting behaviour and influence of climatic variables on the ecology of mosquitoes is also provided.
蚊子（双翅目：蚊科）是常见的吸血双翅目昆虫，常出现在水生环境中，这些环境对许多动物物种，特别是迁徙鸟类来说是有价值的生态系统。因此，这些动物物种与蚊子之间的相互作用可能在病原体传播中起着关键作用。在 2018-2019 年期间，使用不同的方法从西班牙北部的两个水生生态系统中收集了蚊子，并使用经典形态学和分子工具进行了鉴定。使用 CO 诱饵的疾病控制与预防中心（CDC）陷阱和捕蚊网共捕获了 1529 只雄性和雌性 22 种本地蚊子（包括该地区的 8 个新记录）。在吸血的雌性蚊子中，使用 DNA 条形码鉴定出 11 种脊椎动物宿主物种-6 种哺乳动物和 5 种鸟类。在 9 个微生境中确定了 8 种蚊子的发育场所，并捕获了 11 种在人类身上降落的蚊子。蚊子的飞行期因物种而异，有些在春季达到高峰，有些在夏季达到高峰。我们的研究强调了使用各种技术全面描述物种组成和丰度的蚊子采样的优势。还提供了有关营养偏好、叮咬行为以及气候变量对蚊子生态学影响的信息。
