Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Sanya Institute of China Agricultural University, Sanya, 572025, China.
Sci Data. 2023 May 3;10(1):252. doi: 10.1038/s41597-023-02164-5.
Bean flower thrips Megalurothrips usitatus is a staple pest of cowpea and other legumes and causes dramatic economic losses. Its small size allows for easy concealment, and large reproductive capacity easily leads to infestations. Despite the importance of a genome in developing novel management strategies, genetic studies on M. usitatus remain limited. Thus, we generated a chromosome-level M. usitatus genome using a combination of PacBio long read and Hi-C technologies. The assembled genome was 238.14 Mb with a scaffold N50 of 13.85 Mb. The final genome was anchored into 16 pseudo-chromosomes containing 14,000 genes, of which 91.74% were functionally annotated. Comparative genomic analyses revealed that expanded gene families were enriched in fatty acid metabolism and detoxification metabolism (ABC transporters), and contracted gene families were strongly associated with chitin-based cuticle development and sensory perception of taste. In conclusion, this high-quality genome provides an invaluable resource for us to understand the thrips' ecology and genetics, contributing to pest management.
斑潜蝇 Megalurothrips usitatus 是豇豆和其他豆类作物的主要害虫，会造成巨大的经济损失。它的体型小，易于藏身，而且繁殖能力强，很容易大量繁殖。尽管基因组对于开发新型管理策略非常重要，但关于 M. usitatus 的遗传研究仍然有限。因此，我们使用 PacBio 长读长和 Hi-C 技术组合生成了斑潜蝇的染色体水平基因组。组装的基因组大小为 238.14 Mb，支架 N50 为 13.85 Mb。最终的基因组锚定到 16 条假染色体中，包含 14000 个基因，其中 91.74%具有功能注释。比较基因组分析表明，脂肪酸代谢和解毒代谢（ABC 转运蛋白）中富集了扩展的基因家族，而与几丁质基外骨骼发育和味觉感官相关的收缩基因家族则与斑潜蝇的生态和遗传有关，有助于害虫管理。
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