Zhao Jiajia, Wang Zeng, Yin Jiaming, Wei Ying, Li Mingshan, Ma Zhongzheng, Yin Meizhen, Dong Min, Shen Jie, Yan Shuo
Sanya Institute of China Agricultural University, Sanya, 572025, PR China.
State Key Laboratory of Agricultural and Forestry Biosecurity, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
J Nanobiotechnology. 2025 May 20;23(1):366. doi: 10.1186/s12951-025-03460-5.
High resistance of bean flower thrips (BFT, Megalurothrips usitatus) has led to the unscientific application of insecticides to cause famous "toxic cowpea" incidents in China. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays an important role in inducing antioxidant responses and drug detoxification. Therefore, the detoxification genes may be suppressed to control insecticide resistance via Nrf2. Herein, we demonstrated that the expression of most detoxification genes and enzyme activity were remarkably suppressed via nrf2 RNAi. Subsequently, a novel hydrophilic-lipophilic diblock polymer (HLDP) was developed to co-assemble with dsNrf2 and sulfoxaflor (SUL) into nanoscale SUL/HLDP/dsNrf2 complex (221.52 nm). Excitingly, the SUL/HLDP/dsNrf2 complex exhibited excellent leaf adhesion performance, with the smaller contact angle, reduced surface tension, amplified contact area, improved retention, and enhanced plant uptake. Meanwhile, theSUL/HLDP/dsNrf2 displayed high delivery efficiency in vitro and in vivo, and its insecticidal activity against BFTs was significantly higher than SUL. Furthermore, the required doses of SUL/HLDP/dsNrf2 to achieve similar insecticidal activity were 50.14% and 58.42% of SUL via immersion and oral feeding, respectively. Overall, this study elucidated the regulatory role of nrf2 in the detoxification and metabolism of BFTs and developed a self-assembled multicomponent RNA nano-biopesticide to increase the susceptibility of BFTs to insecticides.
豆花蓟马（BFT，豆蓟马）的高抗性导致杀虫剂的不科学施用，在中国引发了著名的“毒豇豆”事件。核因子红细胞2相关因子2（Nrf2）在诱导抗氧化反应和药物解毒中起重要作用。因此，解毒基因可能通过Nrf2被抑制以控制抗药性。在此，我们证明了通过nrf2 RNA干扰，大多数解毒基因的表达和酶活性被显著抑制。随后，开发了一种新型的亲水性-亲脂性双嵌段聚合物（HLDP），使其与dsNrf2和氟吡呋喃酮（SUL）共同组装成纳米级的SUL/HLDP/dsNrf2复合物（221.52纳米）。令人兴奋的是，SUL/HLDP/dsNrf2复合物表现出优异的叶片粘附性能，具有更小的接触角、降低的表面张力、扩大的接触面积、提高的滞留率和增强的植物吸收。同时，SUL/HLDP/dsNrf2在体外和体内均显示出高递送效率，其对豆花蓟马的杀虫活性显著高于SUL。此外，通过浸液法和口服法实现相似杀虫活性所需的SUL/HLDP/dsNrf2剂量分别为SUL的50.14%和58.42%。总体而言，本研究阐明了nrf2在豆花蓟马解毒和代谢中的调控作用，并开发了一种自组装多组分RNA纳米生物农药，以增加豆花蓟马对杀虫剂的敏感性。
