Zhou You, Jiang Dike, Yao Xueping, Luo Yan, Yang Zexiao, Ren Meishen, Zhang Ge, Yu Yuanyuan, Lu Aiping, Wang Yin
Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases (TMBJ), School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
Front Microbiol. 2023 Apr 17;14:1160433. doi: 10.3389/fmicb.2023.1160433. eCollection 2023.
is a gram-negative bacterium that causes fibrotic polyserositis and arthritis in pig, significantly affecting the pig industry. The pan-genome of is open. As the number of genes increases, the core and accessory genomes may show more pronounced differences. The genes associated with virulence and biofilm formation are also still unclear due to the diversity of . Therefore, we have applied a pan-genome-wide association study (Pan-GWAS) to 121 strains . Our analysis revealed that the core genome consists of 1,133 genes associated with the cytoskeleton, virulence, and basic biological processes. The accessory genome is highly variable and is a major cause of genetic diversity in . Furthermore, two biologically important traits (virulence, biofilm formation) of were studied pan-GWAS to search for genes associated with the traits. A total of 142 genes were associated with strong virulence traits. By affecting metabolic pathways and capturing the host nutrients, these genes are involved in signal pathways and virulence factors, which are beneficial for bacterial survival and biofilm formation. This research lays the foundation for further studies on virulence and biofilm formation and provides potential new drug and vaccine targets against .
是一种革兰氏阴性菌，可导致猪的纤维化多浆膜炎和关节炎，对养猪业产生重大影响。的泛基因组是开放的。随着基因数量的增加，核心基因组和辅助基因组可能会表现出更明显的差异。由于的多样性，与毒力和生物膜形成相关的基因也仍不清楚。因此，我们对121株应用了全基因组关联研究（泛基因组全关联研究）。我们的分析表明，核心基因组由1133个与细胞骨架、毒力和基本生物学过程相关的基因组成。辅助基因组高度可变，是遗传多样性的主要原因。此外，通过泛基因组全关联研究对的两个生物学重要性状（毒力、生物膜形成）进行了研究，以寻找与这些性状相关的基因。共有142个基因与强毒力性状相关。通过影响代谢途径和获取宿主营养，这些基因参与信号通路和毒力因子，有利于细菌存活和生物膜形成。这项研究为进一步研究毒力和生物膜形成奠定了基础，并提供了针对的潜在新药物和疫苗靶点。
