Department of Chemical Sciences, McPherson University, Seriki Sotayo, Nigeria.
Department of Biological Sciences, McPherson University, Seriki Sotayo, Nigeria.
Environ Technol. 2024 Jun;45(16):3155-3161. doi: 10.1080/09593330.2023.2210770. Epub 2023 May 15.
This study assessed the low-density polyethylene (LDPE) film degradation potential of microorganisms isolated from oil-contaminated soil and also analyzed the morphological and chemical composition of LDPE films after the biodegradation period. The bacteria strains isolated from oil-contaminated soil were standardized and used to degrade the pretreated LDPE films in mineral salt media. Thereafter, they were incubated for 78 days at 37°C in an incubator shaker, and the degraded LDPE films were analyzed quantitatively and qualitatively (using scanning electron microscope (SEM) images and energy dispersal x-ray (EDX)). Isolates A32 and BTT4 amongst other bacteria isolates showed the highest LDPE film degradation activity, with a weight reduction of 71.80% and 89.72% respectively, and were identified using the 16S rRNA sequencing technique. The EDX results showed that LDPE film incubated with A32 has the highest reduction in carbon and nitrogen (23.8% and 44.9% respectively) when compared with the Control. However, LDPE film incubated with BTT4 had an increase in calcium and chlorine (139% and 40% respectively), when compared with the control. Similarly, the SEM images showed the appearance of pinholes, cracks and particles on the surfaces of LDPE films incubated with A32 and BTT4 contrary to the controls. A32 and BTT4 were identified as (Accession number: MN124173.1) and (Accession number: KY027145.1) respectively. showed viable plastic biodegradation potentials and may be useful in the management of plastic waste, leading to a reduction in global plastic waste and a clean environment.
本研究评估了从受油污染土壤中分离出的微生物对低密度聚乙烯（LDPE）薄膜的降解潜力，并分析了 LDPE 薄膜在生物降解期后的形貌和化学组成。从受油污染土壤中分离出的细菌菌株经过标准化后，用于在无机盐培养基中降解预处理的 LDPE 薄膜。然后，将它们在 37°C 的摇床培养箱中孵育 78 天，并对降解的 LDPE 薄膜进行定量和定性分析（使用扫描电子显微镜（SEM）图像和能量色散 X 射线（EDX））。在其他细菌分离株中，分离株 A32 和 BTT4 表现出最高的 LDPE 薄膜降解活性，分别减少了 71.80%和 89.72%，并使用 16S rRNA 测序技术进行了鉴定。EDX 结果表明，与对照相比，与 A32 孵育的 LDPE 薄膜的碳和氮含量分别降低了 23.8%和 44.9%。然而，与对照相比，与 BTT4 孵育的 LDPE 薄膜的钙和氯含量分别增加了 139%和 40%。同样，SEM 图像显示，与对照相比，与 A32 和 BTT4 孵育的 LDPE 薄膜表面出现了针孔、裂缝和颗粒。A32 和 BTT4 分别被鉴定为 （登录号：MN124173.1）和 （登录号：KY027145.1）。 显示出具有可行的塑料生物降解潜力，可能有助于管理塑料废物，从而减少全球塑料废物并改善环境。
