Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084, Fisciano, Salerno, Italy; UNESCO Chair Salerno, Plantae Medicinales Mediterraneae, University of Salerno, Fisciano, Italy.
Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Baronissi, Italy; UNESCO Chair Salerno, Plantae Medicinales Mediterraneae, University of Salerno, Fisciano, Italy.
J Ethnopharmacol. 2023 Sep 15;313:116579. doi: 10.1016/j.jep.2023.116579. Epub 2023 May 2.
In the Amazon rainforest, the shamans of the Mayantuyacu site use the healing virtues of decoctions and teas from different parts of the Couroupita guianensis Aubl. (Lecythidaceae) trees as remedies in Ashaninka medicine. However, composition of the remedy and the underlying mechanism remain unclear.
This study was designed to compare the metabolite profile of Couroupita guianensis bark decoction produced by Amazonian shamans with that obtained under standardised laboratory conditions and to investigate biological properties of both decoction and isolated constituents in skin wound healing process and inflammation.
The chemical analyses were carried out by Ultra-High-Performance Liquid Chromatography coupled with UV and High-Resolution Mass Spectrometry detectors (UHPLC-UV-HRMS). 1D and 2D-NMR experiments were performed to identify the main decoction constituents. The decoction and pure compound effect on keratinocyte migration was determined by the in vitro wound healing model; the mechanism of action was elucidated by western blot analysis.
UHPLC-UV-HRMS analysis revealed the occurrence of polyphenolic compounds as catechins, ellagitannins and, notably, of unusual sulphated derivatives of ellagic acid isolated for the first time from Couroupita guianensis bark. A new natural sulphated molecule [4-(2″-O-sulphate- β-D-glucuronopyranosyl) ellagic acid] was identified as the potential active compound responsible for the efficacy of bark decoction stimulating wound healing in human HaCaT keratinocytes. The molecular mechanism involved the induction of pro-migratory pathways mediated by ERK and AKT phosphorylation and the increase of MMP2 expression in HaCaT cells. At the same time, the treatment inhibited inflammation interfering with NFkB activation.
Beyond identifying a new bioactive compound, the overall results scientifically validate the traditional use of Couroupita guianensis bark decoction as an anti-inflammatory remedy. Moreover, the beneficial effects on keratinocytes suggest promising therapeutic applications in skin diseases.
在亚马逊雨林，Mayantuyacu 地区的萨满巫师使用 Couroupita guianensis Aubl.（玉蕊科）树木不同部位的汤剂和茶的治疗功效作为 Ashaninka 医学的疗法。然而，该疗法的组成和潜在机制仍不清楚。
本研究旨在比较亚马逊萨满制作的 Couroupita guianensis 树皮汤剂的代谢物谱与在标准化实验室条件下获得的代谢物谱，并研究汤剂和分离成分在皮肤伤口愈合过程和炎症中的生物特性。
通过超高效液相色谱与紫外和高分辨率质谱联用（UHPLC-UV-HRMS）进行化学分析。通过 1D 和 2D-NMR 实验鉴定主要汤剂成分。通过体外伤口愈合模型测定汤剂和纯化合物对角质形成细胞迁移的影响；通过 Western blot 分析阐明作用机制。
UHPLC-UV-HRMS 分析显示多酚类化合物的存在，如儿茶素、鞣花单宁，特别是首次从 Couroupita guianensis 树皮中分离出的鞣花酸的罕见硫酸衍生物。一种新的天然硫酸化分子[4-（2″-O-硫酸-β-D-葡萄糖醛酸吡喃糖苷基）鞣花酸]被鉴定为潜在的活性化合物，负责刺激人 HaCaT 角质形成细胞伤口愈合的树皮汤剂功效。涉及 ERK 和 AKT 磷酸化诱导促迁移途径和 MMP2 在 HaCaT 细胞中表达增加的分子机制。同时，该治疗抑制炎症，干扰 NFkB 激活。
除了鉴定一种新的生物活性化合物外，整体结果从科学上验证了 Couroupita guianensis 树皮汤剂作为抗炎疗法的传统用途。此外，对角质形成细胞的有益作用表明在皮肤病治疗中有有希望的应用。
