Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Laboratório de Farmacologia da Inflamação e do Óxido Nítrico, Brazil.
J Ethnopharmacol. 2013 Mar 7;146(1):324-30. doi: 10.1016/j.jep.2012.12.053. Epub 2013 Jan 11.
Couroupita guianensis Aublet, 'macacarecuia', 'abricó-de-macaco', 'castanha-de-macaco' and 'amêndoa-dos-andes', is found in tropical regions and is widely used in the treatment of tumors, pain, and inflammatory processes.
Ethanol extract and hexane and ethyl acetate fractions were evaluated in models of inflammatory pain (formalin-induced licking) and acute inflammation (carrageenan-induced peritonitis).
Ethanol extract, hexane and ethyl acetate fractions (10, 30 or 100 mg/kg, p.o.) and the reference drugs dexamethasone (5 mg/kg), morphine (5 mg/kg, s.c.), and acetylsalicylic acid (100 mg/kg, p.o.) were tested in formalin-induced licking response and carrageenan-induced peritonitis.
All three doses from Couroupita guianensis fractions significantly reduced the time that the animal spent licking the formalin-injected paw in first and second phases. However, only higher doses (30 and 100 mg/kg) were able to inhibit the leukocyte migration into the peritoneal cavity after carrageenan injection. In this model, the 100 mg/kg dose almost abolished the cell migration. It was also observed that protein concentration resulted from extravasation to the peritoneum and nitric oxide (NO) productions were significantly reduced. Cytokines production was differently affected by the treatment. TNF-α production was reduced after ethanol extract and ethyl acetate fraction pre-treatment whereas hexane fraction had effect only with 100 mg/kg dose. IL-1β production was inhibited only after hexane fraction pre-treatment. The inhibitory effect observed was not due to a direct cytotoxic effect on cells nor to a NO-scavenger activity. The effect was due to a direct inhibition on NO production by the cells.
The results show that Couroupita guianensis fractions have anti-inflammatory effect, partly due to a reduction on cell migration and a inhibition on cytokines and inflammatory mediators production.
Couroupita guianensis Aublet，“macacarecuia”、“abricó-de-macaco”、“castanha-de-macaco”和“amêndoa-dos-andes”，见于热带地区，广泛用于治疗肿瘤、疼痛和炎症过程。
评估乙醇提取物以及己烷和乙酸乙酯馏分在炎症性疼痛（福尔马林诱导舔舐）和急性炎症（角叉菜胶诱导腹膜炎）模型中的作用。
乙醇提取物、己烷和乙酸乙酯馏分（10、30 或 100 mg/kg，po）和参考药物地塞米松（5 mg/kg）、吗啡（5 mg/kg，sc）和乙酰水杨酸（100 mg/kg，po）用于测试福尔马林诱导舔舐反应和角叉菜胶诱导腹膜炎。
Couroupita guianensis 各馏分的三个剂量均显著减少了动物在第一和第二阶段舔注射福尔马林的爪子的时间。然而，只有高剂量（30 和 100 mg/kg）能够抑制角叉菜胶注射后白细胞向腹腔的迁移。在这种模型中，100 mg/kg 剂量几乎完全消除了细胞迁移。还观察到蛋白质浓度由渗出到腹膜和一氧化氮（NO）的产生显著减少。细胞因子的产生受到不同的影响。与预处理相比，乙醇提取物和乙酸乙酯馏分预处理降低了 TNF-α的产生，而己烷馏分仅在 100 mg/kg 剂量时才有作用。IL-1β的产生仅在己烷馏分预处理后受到抑制。观察到的抑制作用不是由于细胞的直接细胞毒性作用或对 NO 的清除活性。该作用是由于细胞对 NO 产生的直接抑制。
结果表明，Couroupita guianensis 馏分具有抗炎作用，部分原因是减少了细胞迁移，并抑制了细胞因子和炎症介质的产生。
