Lee Haeseung, Kwak Dongmi
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Front Vet Sci. 2023 Apr 18;10:1139060. doi: 10.3389/fvets.2023.1139060. eCollection 2023.
(syn. ) is the only species that infects humans and most other mammals. Wild boars are a reservoir of many viruses, bacteria, and parasites that can be transmitted to livestock and humans. This study examined the infection rate of in wild boars and confirmed its specificity by comparing assemblages through PCR amplification of the 18S rRNA, , and β-giardin genes. Fecal samples were collected from roadkilled or trapped wild boars from April 2016 to December 2021 in Korea. DNA was extracted directly from 612 wild boar fecal specimens using a commercial kit. PCR was performed targeting the 18S rRNA region, β-giardin, and glutamate dehydrogenase genes of Some PCR-positive samples were selected for sequencing analysis. The obtained sequences were subsequently used for phylogenetic tree construction. Of the 612 samples tested, 125 (20.4%) were positive for The highest infection rate was detected in the central region (12.0%) and in autumn (12.7%). Among the risk factors, the seasonal factor was statistically significant ( = 0.012). Phylogenetic analysis revealed three genetic assemblages: A, B, and E. Assemblages A and B exhibited 100% identity with sequences isolated from human and farmed pigs in Korea and Japan. This result cannot be ignored because it indicates the possibility of zoonotic transmission. Therefore, continuous management and monitoring of this pathogen are necessary to prevent transmission and protect animal and human health.
（同义词）是唯一感染人类和大多数其他哺乳动物的物种。野猪是许多病毒、细菌和寄生虫的宿主，这些病原体可传播给家畜和人类。本研究检测了野猪中的感染率，并通过对18S rRNA、 （此处原文缺失具体基因名称）和β-贾第虫基因进行PCR扩增来比较菌群，从而确认其特异性。2016年4月至2021年12月期间，从韩国道路上撞死或捕获的野猪中采集粪便样本。使用商业试剂盒直接从612份野猪粪便标本中提取DNA。针对 （此处原文缺失具体物种名称）的18S rRNA区域、β-贾第虫和谷氨酸脱氢酶基因进行PCR。选择一些PCR阳性样本进行测序分析。随后将获得的序列用于构建系统发育树。在检测的612个样本中，125个（20.4%）为 （此处原文缺失具体物种名称）阳性。中部地区（12.0%）和秋季（12.7%）的感染率最高。在风险因素中，季节因素具有统计学意义（ = 0.012）。系统发育分析揭示了三个遗传菌群：A、B和E。菌群A和B与从韩国和日本的人类和养殖猪中分离出的 （此处原文缺失具体物种名称）序列具有100%的同一性。这一结果不容忽视，因为它表明了人畜共患病传播的可能性。因此，有必要对这种病原体进行持续管理和监测，以防止传播并保护动物和人类健康。
