Ardkhongharn Nusara, Ravichotikul Romyakorn, Aksornchai Patthanan, Weluwanarak Thekhawet, Chaiphongpachara Tanawat, Changbunjong Tanasak
Department of Pre-Clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MoZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Int J Parasitol Parasites Wildl. 2023 Apr 20;21:74-82. doi: 10.1016/j.ijppaw.2023.04.008. eCollection 2023 Aug.
The hematophagous flies of the genus Bezzi, 1907 (Diptera: Muscidae) are important ectoparasites in domestic animals and wildlife. Two species of this genus have been recorded in Thailand, viz., (Austen, 1909) and (Pont, Duvallet & Changbunjong, 2020). They have a similar morphology and coexist in the same habitat. The correct species identification of these flies is extremely important for understanding disease epidemiology and developing effective control measures. Geometric morphometrics (GM) has been confirmed to be a useful tool for differentiating and identifying morphologically similar insect species. Therefore, GM was used to distinguish and identify and in Thailand Adult flies of both sexes were collected using Nzi traps, morphologically identified, and analyzed by landmark-based GM of the wing. Results showed that GM was highly effective in distinguishing the two species based on their wing shape, with an overall accuracy score of 99.3%. We also revealed that our study material could be used as reference data to identify new field specimens collected from other geographic locations. We propose that wing GM can be used as a supplement to conventional morphology identification, particularly for specimen that has been damaged or has lost its diagnostic characteristics due to specimen collection and processing in the field.
贝齐属（Bezzi，1907）的吸血蝇（双翅目：蝇科）是家畜和野生动物中重要的体外寄生虫。该属的两个物种已在泰国被记录，即（奥斯汀，1909）和（庞特、杜瓦莱特和昌本宗，2020）。它们形态相似且共存于同一栖息地。对这些苍蝇进行准确的物种鉴定对于理解疾病流行病学和制定有效的控制措施极为重要。几何形态测量学（GM）已被证实是区分和鉴定形态相似昆虫物种的有用工具。因此，GM被用于区分和鉴定泰国的和。使用恩齐诱捕器收集了两性的成年苍蝇，进行形态学鉴定，并通过基于地标点的翅部GM进行分析。结果表明，GM基于翅形在区分这两个物种方面非常有效，总体准确率为99.3%。我们还表明，我们的研究材料可作为参考数据，用于鉴定从其他地理位置采集的新的野外标本。我们建议翅部GM可作为传统形态学鉴定的补充，特别是对于因野外标本采集和处理而受损或失去诊断特征的标本。
