Klaiklueng Nantatchaporn, Kumlert Rawadee, Moonmake Sopavadee, Ruang-Areerate Toon, Siriyasatien Padet, Sunantaraporn Sakone, Wanachiwanawin Darawan, Ruenchit Pichet, Wongkamchai Sirichit
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Division of Vector Borne Diseases, Department of Disease Control, Ministry of Public Health, Nonthaburi, 11000, Thailand.
Curr Res Parasitol Vector Borne Dis. 2025 Apr 21;7:100263. doi: 10.1016/j.crpvbd.2025.100263. eCollection 2025.
Sand flies are principal vectors of spp. and spp. Identifying precise vector species is crucial for effective control. We conducted a study on the species distribution of phlebotomine sand flies in cave-dwelling and non-cave-dwelling in four southern provinces of Thailand. In this study, we collected 621 sand flies (346 females and 275 males) and identified all specimens based on morphology and DNA barcoding, employing cytochrome oxidase subunit 1 (1) and cytochrome () genes. In female specimens, we also screened the small subunit 18S ribosomal RNA (18S rRNA) gene for spp. and spp. Morphologically, 467 (75.2%) sand flies were identified to species level, 47 (7.57%) to subgenus level, and 107 (17.23%) to genus level. These included (43.48%), (26.73%), (2.25%), (2.25%), (0.48%), () spp. (4.83%), () spp. (2.74%), spp. (9.18%), and spp. (8.05%). Among the 107 specimens identified to genus level, DNA barcoding further identified 49 (45.79%) as (1.61%), (0.16%), (2.9%), and (1.61%). No DNA was detected, but DNA was found in females of from Narathiwat Province. Expanding genetic reference databases of sand flies located in four provinces of southern Thailand will improve barcoding accuracy. Understanding sand fly species composition and distribution is imperative for vector control and disease prevention in Thailand.
白蛉是某些物种的主要传播媒介。确定精确的传播媒介物种对于有效控制至关重要。我们对泰国南部四个省份洞穴居住和非洞穴居住环境中的白蛉种类分布进行了一项研究。在这项研究中，我们收集了621只白蛉（346只雌性和275只雄性），并根据形态学和DNA条形码技术，利用细胞色素氧化酶亚基1（COI）和细胞色素b（CYTB）基因对所有标本进行了鉴定。在雌性标本中，我们还筛选了小亚基18S核糖体RNA（18S rRNA）基因以检测某些物种。从形态学上看，467只（75.2%）白蛉被鉴定到物种水平，47只（7.57%）被鉴定到亚属水平，107只（17.23%）被鉴定到属水平。这些包括中华白蛉（43.48%）、微小按蚊（26.73%）、吉氏按蚊（2.25%）、菲律宾按蚊（2.25%）、多斑按蚊（0.48%）、司氏按蚊（4.83%）、大劣按蚊（2.74%）、库蚊属（9.18%）和伊蚊属（8.05%）。在107只被鉴定到属水平的标本中，DNA条形码技术进一步鉴定出49只（45.79%）为致倦库蚊（1.61%）、淡色库蚊（0.16%）、三带喙库蚊（2.9%）和白纹伊蚊（1.61%）。未检测到疟原虫DNA，但在北大年府的微小按蚊雌性个体中发现了疟原虫DNA。扩大泰国南部四个省份白蛉的遗传参考数据库将提高条形码技术的准确性。了解白蛉物种组成和分布对于泰国的病媒控制和疾病预防至关重要。
