Institute of Food Safety and Nutrition, Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Key Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
J Sci Food Agric. 2023 Oct;103(13):6219-6232. doi: 10.1002/jsfa.12690. Epub 2023 May 22.
Mycoplasma hyorhinis is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a large impact on intestinal microecology. To study the effect of M. hyorhinis infection on gut microbial composition and metabolome profile, pigs were infected with M. hyorhinis. Metagenomic sequencing analysis was performed of fecal samples and a liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis of gut digesta was made.
Pigs infected with M. hyorhinis had enriched Sutterella and Mailhella, and depleted Dechloromonas, Succinatimonas, Campylobacter, Blastocystis, Treponema, and Megasphaera. The pigs infected with M. hyorhinis also had greater abundances of bacterium_0_1xD8_71, Ruminococcus_sp__CAG_353, Firmicutes_bacterium_CAG_194, Firmicutes_bacterium_CAG_534, bacterium_1xD42_87, and lower abundances of Chlamydia_suis, Megasphaera_elsdenii, Treponema_porcinum, Bacteroides_sp__CAG_1060, Faecalibacterium_prausnitzii. Metabolomic analysis revealed that some lipids and lipid-like molecules increased in the small intestine, whereas most lipids and lipid-like molecule metabolites decreased in the large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism, and thiamine metabolism.
These findings demonstrate that infection with M. hyorhinis can alter the gut microbial composition and metabolite structure in pigs, which may further affect amino acid metabolism and lipid metabolism in the intestine. © 2023 Society of Chemical Industry.
猪支原体是一种常见的猪呼吸道病原体，给养猪生产者造成了巨大的经济损失。越来越多的证据表明，呼吸道病原体感染对肠道微生物群有很大影响。为了研究猪支原体感染对肠道微生物组成和代谢组谱的影响，本研究用猪支原体感染猪。对粪便样本进行宏基因组测序分析，并对肠道内容物进行液相色谱/串联质谱（LC-MS/MS）分析。
感染猪支原体的猪富集了 Sutterella 和 Mailhella，而耗竭了 Dechloromonas、Succinatimonas、Campylobacter、Blastocystis、Treponema 和 Megasphaera。感染猪支原体的猪还具有更高丰度的 bacterium_0_1xD8_71、Ruminococcus_sp__CAG_353、Firmicutes_bacterium_CAG_194、Firmicutes_bacterium_CAG_534、bacterium_1xD42_87 和更低丰度的 Chlamydia_suis、Megasphaera_elsdenii、Treponema_porcinum、Bacteroides_sp__CAG_1060、Faecalibacterium_prausnitzii。代谢组学分析表明，一些在小肠中增加的脂质和类脂分子，而大多数在大肠中减少的脂质和类脂分子代谢物。这些改变的代谢物诱导肠道鞘脂代谢、氨基酸代谢和硫胺素代谢的变化。
这些发现表明，猪支原体感染可以改变猪的肠道微生物组成和代谢产物结构，这可能进一步影响肠道中的氨基酸代谢和脂质代谢。 © 2023 化学工业协会。
