Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, DF, Brazil.
Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis, IBAMA, Brasília, DF, Brazil.
Methods Mol Biol. 2023;2667:129-138. doi: 10.1007/978-1-0716-3199-7_10.
The experimental rodent models for the fungal disease are a handy tool for understanding host-fungus interactions. To Fonsecaea sp., one of the causative agents of chromoblastomycosis, there is an extra challenge because the animals preferably used show a spontaneous cure; so until now, there is no model to reproduce the long-term disease similar to human chronic disease. In this chapter, we described an experimental model using rats and mice with a subcutaneous route, with the checkpoints of acute-like and chronic-like lesion analysis comparable with human lesions, the fungal burden, and the lymphocytes investigation.
真菌病的实验啮齿动物模型是了解宿主-真菌相互作用的便捷工具。对于引起着色芽生菌病的病原菌之一的外瓶霉属，存在一个额外的挑战，因为首选的动物通常会自发治愈；因此，到目前为止，还没有可复制类似于人类慢性疾病的长期疾病的模型。在本章中，我们描述了一种使用皮下途径的大鼠和小鼠的实验模型，具有类似于人类病变的急性和慢性病变分析、真菌负荷和淋巴细胞研究的检查点。
