Bodnar R J, Truesdell L S, Haldar J, Aral I A, Kordower J H, Nilaver G
Peptides. 1986 Jan-Feb;7(1):111-7. doi: 10.1016/0196-9781(86)90070-7.
Pain thresholds are increased following central administration of arginine vasopressin (AVP), an effect which appears not to be mediated through opioid analgesic processes. In addition to magnocellular projections to the posterior lobe of the pituitary gland and parvocellular projections to the zona externa of the median eminence, the paraventricular nucleus (PVN) of the hypothalamus contains VP parvocellular neurons which also project to extrahypothalamic structures involved in pain inhibition. The present study examined whether AVP analgesia as measured by the tail-flick test was altered in animals with lesions placed in the PVN at either 7 or 35 days after surgery. VP levels in the pons-medulla and the lumbo-sacral spinal cord were measured by radioimmunoassay, as well as VP-like immunoreactivity in the PVN and spinal cord with immunocytochemistry. Lesions placed in the PVN eliminated AVP analgesia on the tail-flick test at both 7 and 35 days after surgery, and decreased radioimmunoassayable VP by 59% in the lumbo-sacral spinal cord and 36% in the pons-medulla. The extent of the lesions ranged from complete destruction of the PVN to partial sparing of ventro-medial PVN cells with VP-like immunoreactivity. These data indicate that the PVN is a critical structure for the integrity of AVP analgesia.
在中枢给予精氨酸加压素（AVP）后，痛阈会升高，这种效应似乎不是通过阿片类镇痛过程介导的。除了下丘脑室旁核（PVN）有向垂体后叶的大细胞投射以及向正中隆起外侧带的小细胞投射外，PVN还含有向参与痛觉抑制的下丘脑外结构投射的VP小细胞神经元。本研究检测了在术后7天或35天对PVN进行损伤的动物中，通过甩尾试验测量的AVP镇痛作用是否发生改变。采用放射免疫分析法测量脑桥-延髓和腰骶脊髓中的VP水平，并用免疫细胞化学方法检测PVN和脊髓中的VP样免疫反应性。在术后7天和35天，对PVN进行损伤均消除了甩尾试验中的AVP镇痛作用，并使腰骶脊髓中可通过放射免疫分析检测到的VP减少59%，脑桥-延髓中减少36%。损伤程度从PVN的完全破坏到腹内侧PVN细胞部分保留VP样免疫反应性不等。这些数据表明，PVN是AVP镇痛完整性的关键结构。
