Praeger F C, Gilchrest B A
Proc Soc Exp Biol Med. 1986 Jul;182(3):315-21. doi: 10.3181/00379727-182-42345.
Elevated calcium chloride concentration [( CaCl2]) has been shown to increase saturation density for an established mouse fibroblast line and for human fetal lung fibroblasts (WI-38). In order to examine the effect of increased [CaCl2] on human fibroblasts from donors of varying age, fibroblasts were grown in medium (basal level of 1.8 mM CaCl2) supplemented with fetal bovine serum (FBS) until confluent. Compared to controls in basal medium, newborn foreskin fibroblasts exposed to additional CaCl2 had a 110-450% increased cell yield that was independent of [CaCl2] within the range of an additional 1.5-5.0 mM. The effect was maintained over an eightfold range of FBS concentration. Initial growth rate was unaffected, but a prolongation of exponential phase occurred for cultures exposed to increased [CaCl2]. Confluent cultures refed medium with increased [CaCl2] were stimulated 5- to 10-fold more than cultures refed basal medium. An additional 2 mM CaCl2 resulted in a 210% increase for young adult-derived fibroblasts versus a 29% increase for old adult-derived fibroblasts (P less than 0.001). These data indicate that increased [CaCl2] decreases density-dependent growth inhibition of postnatal human dermal fibroblasts in vitro and that this effect is donor age dependent.
氯化钙浓度升高（[CaCl₂]）已被证明可提高已建立的小鼠成纤维细胞系和人胎儿肺成纤维细胞（WI-38）的饱和密度。为了研究[CaCl₂]升高对不同年龄供体的人成纤维细胞的影响，将成纤维细胞在补充有胎牛血清（FBS）的培养基（基础水平为1.8 mM CaCl₂）中培养至汇合。与基础培养基中的对照相比，暴露于额外CaCl₂的新生儿包皮成纤维细胞的细胞产量增加了110 - 450%，在额外1.5 - 5.0 mM的范围内，该增加与[CaCl₂]无关。该效应在FBS浓度的八倍范围内得以维持。初始生长速率未受影响，但暴露于升高的[CaCl₂]的培养物的指数生长期延长。用升高[CaCl₂]的培养基重新培养的汇合培养物比用基础培养基重新培养的培养物受到的刺激多5至10倍。额外添加2 mM CaCl₂导致年轻成人来源的成纤维细胞增加210%，而老年成人来源的成纤维细胞增加29%（P小于0.001）。这些数据表明，[CaCl₂]升高可降低体外出生后人皮肤成纤维细胞的密度依赖性生长抑制，且这种效应依赖于供体年龄。
