Praeger F C, Cristofalo V J
In Vitro Cell Dev Biol. 1986 Jun;22(6):355-9. doi: 10.1007/BF02623410.
The growth of WI-38 cells in serum-free growth medium with and without hormone supplementation in the presence of elevated Ca2+ concentrations was investigated. At 5 mM CaCl2, WI-38 cells seeded at low density without serum or hormone supplementation showed up to a 12-fold increase in cell number at saturation density over that obtained at day 1. Saturation densities were comparable when either 5 mM CaCl2 or epidermal growth factor (1 mM CaCl2) was used in the presence of insulin, dexamethasone and transferrin. Combining suboptimal doses of epidermal growth factor and CaCl2 resulted in an additive effect on saturation density. Thus, normal human diploid cells are capable of substantial growth in serum-free, hormone-free growth medium. In contrast, confluent cultures refed with the same medium are not responsive to elevated Ca2+ concentrations. In fact, elevated Ca2+ concentrations inhibited the proliferative response of confluent cultures to epidermal growth factor, but enhanced their response to the combined treatment of insulin, transferrin and dexamethasone.
研究了WI-38细胞在添加和不添加激素的无血清生长培养基中，在Ca2+浓度升高的情况下的生长情况。在5 mM CaCl2条件下，低密度接种且不添加血清或激素的WI-38细胞在饱和密度时的细胞数量比第1天增加了12倍。在胰岛素、地塞米松和转铁蛋白存在的情况下，使用5 mM CaCl2或表皮生长因子（1 mM CaCl2）时，饱和密度相当。次优剂量的表皮生长因子和CaCl2联合使用对饱和密度产生累加效应。因此，正常人二倍体细胞能够在无血清、无激素的生长培养基中大量生长。相比之下，用相同培养基重新培养的汇合培养物对升高的Ca2+浓度没有反应。事实上，升高的Ca2+浓度抑制了汇合培养物对表皮生长因子的增殖反应，但增强了它们对胰岛素、转铁蛋白和地塞米松联合处理的反应。
