School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.
College of Traditional Chinese Medicine?College of Intergrated Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China.
J Tradit Chin Med. 2023 Jun;43(3):507-513. doi: 10.19852/j.cnki.jtcm.20230214.004.
To observe the efficacy of Danggui Buxue decoction (, DBD) on diabetic nephropathy-induced renal fibrosis in rats, and to study the possible mechanism.
Sixty male Goto Kakizaki (GK) rats were randomly assigned to the model group, gliquidone group, astragaloside IV group, and high-, medium- and low-doses DBD groups. After 8 weeks, changes in body weight, blood glucose, serum creatinine, serum urea nitrogen, and total cholesterol were observed. Changes in transforming growth factor-β1 (TGF-β1), Smad3, and Smad5 pathways and the expression of the fibrosis-related proteins collagen IV (col IV), α-smooth muscle actin (α-SMA), and vimentin were assessed. The degree of renal fibrosis was observed by immunohistochemistry and Mason staining. The expression of interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor (TNF-α), and C-reactive protein (CRP) in the kidneys was assessed using enzyme linked immunosorbent assay.
Our experiments showed that DBD effectively reduced blood glucose, blood urea nitrogen, and creatinine levels after 8 weeks of administration, improved renal function in diabetic rats, alleviated renal fibrosis, and reduced the renal tissue levels of IL-6, IL-10, TNF-α, and CRP. Furthermore, DBD decreased the expression of TGF-β1, Smad3, col IV, α-SMA, and vimentin in renal tissues and increased the expression of Smad5.
DBD ameliorates diabetic renal interstitial fibrosis by modulating the TGF-β1/Smads pathway.
观察当归补血汤对糖尿病肾病大鼠肾纤维化的疗效，并探讨其可能的作用机制。
将 60 只雄性 GK 大鼠随机分为模型组、格列喹酮组、黄芪甲苷 IV 组及当归补血汤高、中、低剂量组。8 周后，观察各组大鼠体质量、血糖、血肌酐、血尿素氮、总胆固醇的变化；检测转化生长因子-β1（TGF-β1）、Smad3、Smad5 通路及纤维化相关蛋白Ⅳ型胶原（col IV）、α-平滑肌肌动蛋白（α-SMA）、波形蛋白的表达；免疫组化法和Mason 染色观察肾脏纤维化程度；酶联免疫吸附法检测肾脏组织中白细胞介素 6（IL-6）、白细胞介素 10（IL-10）、肿瘤坏死因子-α（TNF-α）和 C 反应蛋白（CRP）的表达。
实验结果显示，当归补血汤可有效降低大鼠 8 周后的血糖、血尿素氮和血肌酐水平，改善糖尿病大鼠的肾功能，减轻肾脏纤维化，降低肾脏组织中 IL-6、IL-10、TNF-α 和 CRP 的表达，下调 TGF-β1、Smad3、col IV、α-SMA 和波形蛋白的表达，上调 Smad5 的表达。
当归补血汤通过调控 TGF-β1/Smads 通路改善糖尿病肾间质纤维化。
