P16、Ki-67 和 CD44 在宫颈高级别鳞状上皮内瘤变和鳞状细胞癌中的表达及相关性研究。

Association of P16, Ki-67, and CD44 expression in high-grade squamous intraepithelial neoplasia and squamous cell carcinoma of the cervix.

机构信息

Department of Pathology, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

Department of Obstetrics and Gynecology, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

出版信息

J Cancer Res Ther. 2023 Apr;19(Supplement):S260-S267. doi: 10.4103/jcrt.jcrt_43_21.

DOI:10.4103/jcrt.jcrt_43_21

PMID:37148002

Abstract

BACKGROUND

Stem cells exist in niches in the cervical tissue at squamocolumnar junction, which when infected with HR-Human Papilloma Virus undergo malignant transformation to cancer stem cells and have a role in carcinogenesis and metastasis. The expression of CD44, P16, and Ki67 in high-grade squamous intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC) is assessed in this study.

MATERIALS AND METHODS

Twenty-six cases each of normal cervix, HSIL, and SCC of cervix cases were subjected to immunohistochemistry markers; p16, Ki-67, and CD44. The association of expression of these markers between normal, HSIL, SCC cervix, and clinic-pathological parameters was statistically analyzed. P < 0.05 was considered significant.

RESULTS

Of 26 cases of HSIL, 61.5%, 7.7%, and 30.8% cases were positive, ambiguous, and negative respectively for p16 expression. About 11.5%, 53.8%, and 34.6% of cases were strongly positive, positive, and weakly positive, respectively, for Ki-67 expression. About 42.3%, 42.3%, and 15.4% cases were strongly positive, positive, and weakly positive, respectively, for CD44 expression. Among 26 cases of SCC of the cervix 92.3% and 7.7% were positive and ambiguous respectively. About 73.1% and 26.9% of cases were strongly positive and positive, respectively, for Ki-67 expression. 65.4%, 30.8%, and 3.8% of cases were strongly positive, positive, and weakly positive, respectively, for CD44 expression. p16, Ki-67, and CD44 expression between the three groups were statistically significant. p16 expression versus FIGO stage including lymph node involvement and CD44 expression versus lymph node involvement in carcinoma cervix was statistically significant.

CONCLUSION

Expression of p16, Ki-67, and CD44 increases as the lesion progress from normal to HSIL to carcinoma cervix. p16 and CD44 expression increase with lymph node involvement. P16 expression was maximum in Stage II than Stage III.

摘要

背景

干细胞存在于宫颈组织的柱状上皮与鳞状上皮交界处的龛位中，当感染人乳头瘤病毒（HPV）时，它们会发生恶性转化为癌症干细胞，并在致癌和转移中起作用。本研究评估了高等级鳞状上皮内病变（HSIL）和宫颈鳞状细胞癌（SCC）中 CD44、P16 和 Ki67 的表达。

材料和方法

对 26 例正常宫颈、HSIL 和 SCC 宫颈病例进行免疫组织化学标志物 p16、Ki-67 和 CD44 检测。对这些标志物在正常、HSIL、SCC 宫颈与临床病理参数之间的表达进行统计学分析。P<0.05 为有统计学意义。

结果

26 例 HSIL 中，p16 表达阳性、不确定和阴性的比例分别为 61.5%、7.7%和 30.8%。Ki-67 表达强阳性、阳性和弱阳性的比例分别为 11.5%、53.8%和 34.6%。CD44 表达强阳性、阳性和弱阳性的比例分别为 42.3%、42.3%和 15.4%。26 例 SCC 中，阳性和不确定的比例分别为 92.3%和 7.7%。Ki-67 表达强阳性和阳性的比例分别为 73.1%和 26.9%。CD44 表达强阳性、阳性和弱阳性的比例分别为 65.4%、30.8%和 3.8%。三组间 p16、Ki-67 和 CD44 的表达差异有统计学意义。p16 表达与 FIGO 分期（包括淋巴结受累）和 SCC 中 CD44 表达与淋巴结受累之间有统计学意义。