Department of Surgery, Pittsburgh Veterinary Specialty & Emergency Center-BluePearl, Pittsburgh, Pennsylvania, USA.
Musculoskeletal Oncology Laboratory, Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Vet Comp Oncol. 2023 Sep;21(3):559-564. doi: 10.1111/vco.12903. Epub 2023 May 6.
Twenty-four dogs with OS underwent limb amputation. Serum, OS tumour, and normal bone were harvested at time of surgery. RNA was extracted and gene expression was performed using quantitative polymerase chain reaction (qPCR). Tissue and blood copper concentrations were also determined with spectrophotometry. Compared to bone, tumour samples had significantly higher expressions of antioxidant 1 copper chaperone (ATOX1, p = .0003). OS tumour copper levels were significantly higher than that of serum (p < .010) and bone (p = .038). Similar to our previous observations in mouse and human OS, dog OS demonstrates overexpression of genes that regulate copper metabolism (ATOX1), and subsequent copper levels. Dogs with OS may provide a robust comparative oncology platform for the further study of these factors, as well as potential pharmacologic interventions.
二十四只患有骨肉瘤的狗接受了截肢手术。在手术时采集了血清、骨肉瘤肿瘤和正常骨骼样本。使用定量聚合酶链反应 (qPCR) 提取 RNA 并进行基因表达分析。还使用分光光度法测定组织和血液中的铜浓度。与骨骼相比，肿瘤样本中抗氧化 1 铜伴侣（ATOX1）的表达显著更高（p =.0003）。骨肉瘤肿瘤中的铜水平明显高于血清（p <.010）和骨骼（p =.038）。与我们之前在小鼠和人类骨肉瘤中的观察结果相似，狗的骨肉瘤表现出调节铜代谢（ATOX1）和随后的铜水平的基因过度表达。患有骨肉瘤的狗可能为进一步研究这些因素以及潜在的药物干预提供一个强大的比较肿瘤学平台。
