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海洋蓝藻鱼腥藻和束丝藻的过氧化氢胁迫。

Hydrogen Peroxide Stress Induced in the Marine Cyanobacterium Synechococcus aeruginosus and Phormidium valdarianum.

机构信息

Department of Botany, J. J. College of Arts and Science (Autonomous), Pudukkottai, 622422, Tamil Nadu, India.

Department of Botany, Srimad Andavan Arts and Science College (Autonomous), Tiruchirappalli, Tamil Nadu, India.

出版信息

Appl Biochem Biotechnol. 2024 Jan;196(1):522-536. doi: 10.1007/s12010-023-04504-y. Epub 2023 May 6.

Abstract

Biochemical markers against hydrogen peroxide-induced oxidative stress were developed in marine cyanobacteria under standard laboratory conditions. To find out the ability to cope with different concentrations of hydrogen peroxide, two species of marine cyanobacteria including unicellular and filamentous forms were exposed for shorter duration. Synechococcus aeruginosus and Phormidium valderianum tolerated hydrogen peroxide by showing the highest growth of Superoxide dismutase in Synechococcus aeruginosus and Phormidium valderianum, catalase in Synechococcus aeruginosus, peroxidase in Synechococcus aeruginosus and Phormidium valderianum, Glutathione S-transferase in Synechococcus aeruginosus and Phormidium valderianum which were identified as biochemical markers of oxidative stress against HO in marine cyanobacteria. Synechococcus aeruginosus showed new isoforms for Superoxide dismutase, catalase, peroxidase, Glutathione peroxidase, and Glutathione S-transferase and Phormidium valderianum for Superoxide dismutase, peroxidase, and Glutathione S-transferase. Synechococcus aeruginosus is suggested as the indicator species for biochemical markers against hydrogen peroxide in marine cyanobacteria. Peroxidase is suggested as biochemical enzyme marker. The present investigated on these new isoenzymes were identified as biochemical markers for oxidative stress.

摘要

在标准实验室条件下,开发了用于海洋蓝藻的针对过氧化氢诱导的氧化应激的生化标志物。为了找出应对不同浓度过氧化氢的能力,对两种海洋蓝藻(包括单细胞和丝状形式)进行了较短时间的暴露。鱼腥藻和钝顶螺旋藻通过表现出鱼腥藻和钝顶螺旋藻中超氧化物歧化酶、鱼腥藻中的过氧化氢酶、鱼腥藻和钝顶螺旋藻中的过氧化物酶、鱼腥藻和钝顶螺旋藻中的谷胱甘肽 S-转移酶的最高生长,耐受了过氧化氢,这些酶被鉴定为海洋蓝藻中针对 HO 的氧化应激的生化标志物。鱼腥藻显示出超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶的新同工酶,钝顶螺旋藻显示出超氧化物歧化酶、过氧化物酶和谷胱甘肽 S-转移酶的新同工酶。鱼腥藻被建议作为海洋蓝藻中针对过氧化氢的生化标志物的指示物种。过氧化物酶被建议作为生化酶标志物。对这些新同工酶的研究被鉴定为氧化应激的生化标志物。

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