Nivedha Ravi Mourthy, Prasanna Radha
Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Arch Microbiol. 2025 Aug 30;207(10):246. doi: 10.1007/s00203-025-04449-2.
Cyanobacteria interact with biota, including higher plants, through the secretion of metabolites, which provide nutrition or elicit immunity, thereby establishing beneficial linkages. However, the phytochemical diversity, in relation to their plant-growth promoting and biofertilizing roles is less understood. Towards this endeavour, the metabolite profiles of three cyanobacterial cultures-Anabaena laxa (C11), Nostoc carneum (BF2), and Anabaena doliolum (BF4)-were evaluated. Biochemical attributes were analysed at 14 and 21 DAI (Days After Inoculation), followed by untargeted GC-MS (Gas Chromatography-Mass Spectrometry) analyses of the metabolite profiles at 21 DAI. Partial Least Squares Discriminant Analysis (PLS-DA), supported by Volcano plot and Correlation network analyses, were employed to highlight the key metabolites. Partial Least Squares Discriminant Analysis (PLS-DA), supported by Volcano plot and Correlation network analyses, illustrated the predominance of organic compounds, in particular, sugars, amino acids and their conjugates, illustrating the significance of C-N metabolism in all the cultures. The metabolites were also rich in response molecules to environmental stressors and a multiplicity of bioactive molecules (particularly glyceryl glycoside, glycerol, lactose, sucrose, and glutamic acid). A. laxa yielded the most hits across several chemical classes, including sugars, organic compounds, amino acids, and nucleotides. Comparison with previously documented metabolite profiles highlighted the differential relative abundance of these compounds, representative of the core metabolome of these agriculturally-significant cyanobacteria. This phytochemical-analysis guided investigation provided insights into the biostimulatory role of cyanobacterial metabolites in nutritional and signalling mechanisms, underscoring their significance as biological options for boosting crop growth and soil fertility.
蓝藻细菌通过分泌代谢物与包括高等植物在内的生物群落相互作用，这些代谢物提供营养或引发免疫反应，从而建立有益的联系。然而，关于它们促进植物生长和生物施肥作用的植物化学多样性，人们了解较少。为了实现这一目标，对三种蓝藻细菌培养物——疏松鱼腥藻（C11）、肉色念珠藻（BF2）和束丝藻（BF4）的代谢物谱进行了评估。在接种后14天和21天分析生化特性，然后在接种后21天对代谢物谱进行非靶向气相色谱 - 质谱（GC - MS）分析。采用偏最小二乘判别分析（PLS - DA），并辅以火山图和相关网络分析，以突出关键代谢物。偏最小二乘判别分析（PLS - DA），在火山图和相关网络分析的支持下，表明有机化合物占主导地位，特别是糖类、氨基酸及其共轭物，这说明了碳氮代谢在所有培养物中的重要性。这些代谢物还富含对环境应激源的响应分子和多种生物活性分子（特别是甘油糖苷、甘油、乳糖、蔗糖和谷氨酸）。疏松鱼腥藻在多个化学类别中产生的命中数最多，包括糖类、有机化合物、氨基酸和核苷酸。与先前记录的代谢物谱进行比较，突出了这些化合物的相对丰度差异，代表了这些具有农业重要性的蓝藻细菌的核心代谢组。这种植物化学分析指导的研究为蓝藻细菌代谢物在营养和信号传导机制中的生物刺激作用提供了见解，强调了它们作为促进作物生长和土壤肥力的生物选择的重要性。
