植物细胞壁的仿生聚合物组装体，用于通过 FRAP 测量蛋白-碳水化合物相互作用。
Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP.
机构信息
Fractionation of AgroResources and Environment (FARE) laboratory, INRAE, Université de Reims Champagne-Ardenne, Reims, France.
出版信息
Methods Mol Biol. 2023;2657:151-162. doi: 10.1007/978-1-0716-3151-5_11.
The interactions of proteins involved in plant cell wall hydrolysis, such as enzymes and CBMs, significantly determine their role and efficiency. In order to go beyond the characterization of interactions with simple ligands, bioinspired assemblies combined with the measurement of diffusion and interaction by FRAP offer a relevant alternative for highlighting the importance of different parameters related to the protein affinity and to the polymer type and organization in the assembly.
参与植物细胞壁水解的蛋白质（如酶和 CBMs）之间的相互作用，显著决定了它们的作用和效率。为了超越与简单配体相互作用的特征描述，生物启发组装结合 FRAP 测量扩散和相互作用提供了一个相关的替代方案，用于突出与蛋白质亲和力以及聚合物类型和组装组织相关的不同参数的重要性。
Zotero 插件