Department of Chemical Engineering and Biotechnology, National Taipei University of Technology. 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan.
Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, No. 5, Fuxing St., Taoyuan 333, Taiwan; College of Medicine, Chang Gung University, No. 259, Wenhua 1st Rd., Taoyuan 333, Taiwan.
Cont Lens Anterior Eye. 2023 Aug;46(4):101852. doi: 10.1016/j.clae.2023.101852. Epub 2023 May 5.
To understand the effects of long-term deposition of tear film components on the surface and optical properties of orthokeratology (ortho-k) lenses, two different lenses, Brighten 22 and Optimum Extra, were tested here.
Ortho-k lenses were immersed in artificial tears and cleaned with a commercial care solution repeatedly for up to 90 days. Both the daily and accumulated lysozyme deposition amounts using an Enzyme-Linked ImmunoSorbent Assay were then analyzed. The base curve, central thickness, power, and transmission of visible light, ultraviolet A, and ultraviolet B were analyzed before and after repeated tear film component deposition procedures. The surface roughness using atomic force microscopy was observed and an energy dispersive spectrometer was used to analyze the composition of the deposits.
The highest levels of lysozyme were adsorbed on both lens materials during the first four days of the procedure and became saturated by day 6. For both lens materials, contamination on the lenses was easily observed by day 30, and the degree of surface roughness was higher. The transmission levels of different light spectrums were reduced showing that the optical characteristics of both lenses were also affected.
The results provide in vitro evidence that lysozyme could not be completely removed from orthokeratology lenses. Both surface and optical properties were affected by the deposition of tear film components. However, only one commercial multipurpose care solution was used to clean the lens in this study when the main ingredient was a surfactant, and the results might be different when other care regimens with other key ingredients are used. In addition, whether tear film component deposition might result in increased risks of infection or corneal abrasion will require further investigation.
为了了解泪膜成分长期沉积对角膜塑形术（ortho-k）镜片表面和光学性能的影响，本研究对两种不同的镜片，Brighten 22 和 Optimum Extra 进行了测试。
将 ortho-k 镜片反复浸入人工泪液和市售护理液中，最长达 90 天。然后使用酶联免疫吸附试验（Enzyme-Linked ImmunoSorbent Assay）分析每日和累积溶菌酶沉积量。分析了重复泪膜成分沉积程序前后的基弧、中心厚度、屈光度和可见光、紫外线 A 和紫外线 B 的透过率。使用原子力显微镜观察表面粗糙度，并使用能量色散光谱仪分析沉积物的成分。
在程序的前四天，两种镜片材料上吸附的溶菌酶水平最高，并在第 6 天达到饱和。对于两种镜片材料，在第 30 天左右很容易观察到镜片上的污染，表面粗糙度也更高。不同光谱的透过率降低，表明两种镜片的光学特性也受到影响。
结果提供了体外证据，表明溶菌酶不能从角膜塑形术镜片上完全去除。泪膜成分的沉积会影响镜片的表面和光学性能。然而，本研究仅使用一种含有表面活性剂的市售多功能护理液来清洁镜片，当使用其他含有其他关键成分的护理方案时，结果可能会有所不同。此外，泪膜成分的沉积是否会导致感染或角膜擦伤的风险增加，还需要进一步研究。
