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抗嗜酸性粒细胞抗体单独或与抗中性粒细胞胞质抗体(ANCA)在不同自身免疫性疾病中的检测。

Antieosinophil Antibodies Alone or in Combination with Antineutrophil Cytoplasmic Antibodies (ANCA) Detected in Different Autoimmune Conditions.

机构信息

Department of Rheumatology and Inflammation Research, Institute of Medicine at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Clinical Immunology and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden.

出版信息

J Immunol Res. 2023 Apr 26;2023:5980287. doi: 10.1155/2023/5980287. eCollection 2023.

Abstract

Circulating antieosinophil antibodies (AEOSA) have been associated with various autoimmune conditions affecting the liver, kidneys, lungs, and joints but are not part of routine clinical diagnostics. While analyzing human sera for antineutrophil cytoplasmic antibodies (ANCA) by indirect immunofluorescence (IIF) on granulocytes, 0.8% of analyzed samples were found to be reactive with eosinophils. Our aim was to determine the diagnostic relevance and antigenic specificity of AEOSA. AEOSA were seen either in combination with an myeloperoxidase (MPO)-positive p-ANCA (44%; AEOSA+/ANCA+) or on their own (56%; AEOSA+/ANCA-). AEOSA/ANCA positivity was seen in patients with thyroid disease (44%) or vasculitis (31%), while AEOSA+/ANCA- pattern was more common in patients with autoimmune disorders of the gastrointestinal tract and/or liver. Eosinophil peroxidase (EPX) was the main target recognized in 66% of the AEOSA+ sera by enzyme-linked immunosorbent assay (ELISA). Eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin (EDN) were also identified as target antigens but less frequently and only in combination with EPX. In conclusion, we confirmed that EPX is a major target of AEOSA, illustrating the high antigenic potential of EPX. Our results also demonstrate the presence of concomitant AEOSA/ANCA positivity in a defined patient group. Further research should aim to elucidate the association of AEOSA with autoimmunity.

摘要

循环抗嗜酸性粒细胞抗体(AEOSA)与影响肝脏、肾脏、肺部和关节的各种自身免疫性疾病有关,但不是常规临床诊断的一部分。在通过间接免疫荧光(IIF)在粒细胞上分析人类血清中的抗中性粒细胞细胞质抗体(ANCA)时,发现 0.8%的分析样本与嗜酸性粒细胞反应。我们的目的是确定 AEOSA 的诊断相关性和抗原特异性。AEOSA 要么与髓过氧化物酶(MPO)阳性 p-ANCA 一起出现(44%;AEOSA+/ANCA+),要么单独出现(56%;AEOSA+/ANCA-)。AEOSA/ANCA 阳性见于甲状腺疾病(44%)或血管炎(31%)患者,而 AEOSA+/ANCA-模式在胃肠道和/或肝脏自身免疫性疾病患者中更为常见。酶联免疫吸附试验(ELISA)显示,66%的 AEOSA+血清中主要识别到过氧化物酶(EPX)。还鉴定出嗜酸性粒细胞阳离子蛋白(ECP)和嗜酸性粒细胞衍生的神经毒素(EDN)作为靶抗原,但频率较低,仅与 EPX 联合使用。总之,我们证实 EPX 是 AEOSA 的主要靶标,说明了 EPX 的高抗原性潜力。我们的结果还表明,在定义明确的患者群体中存在同时存在的 AEOSA/ANCA 阳性。进一步的研究应旨在阐明 AEOSA 与自身免疫的关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab0/10156452/265b4049cd39/JIR2023-5980287.001.jpg

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