Li Zong-Yu, Li Xiao-Lin, Wei Peng-Gong, Qu Liu, DU Li-Hong, Yu Ya-Qiong
Department of Endodontics, School and Hospital of Stomatology, China Medical University; Liaoning Provincial Key Laboratory of Oral Diseases. Shenyang 110002, Liaoning Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2023 Apr;32(2):132-136.
To investigate whether resveratrol promotes odontogenic differentiation of human dental pulp stem cells(DPSCs) by up-regulating the expression of silent information regulator 1 (SIRT1) and activating β-catenin signaling pathway.
Different concentrations of resveratrol(0, 10, 15, 20 and 50 μmol/L) were used to treat DPSCs for 7 days and 14 days, and cell proliferative activity was detected by CCK-8. After odontogenic differentiation induced by 15 μmol/L resveratrol for 7 days, alkaline phosphatase(ALP) staining was performed and real-time quantitative reverse transcription PCR(qRT-PCR) was used to detect the mRNA expression of Runt-related transcription factor 2 (Runx2), dentin sialophosphoprotein(DSPP) and dentin matrix protein-1(DMP-1) in DPSCs. Western blot was used to detect the expression of SIRT1 in DPSCs on a specific day (0, 3rd, 5th, 7th and 14th) after differentiation induction. Western blot was also used to detect the expression of SIRT1 and activated β-catenin during odontogenic differentiation of DPSCs treated by 15 μmol/L resveratrol for 7 days. The experimental data was analyzed with GraphPad Prism 9 software package.
15 μmol/L resveratrol had no significant effect on proliferation of DPSCs on the 7th and 14th day; 15 μmol/L resveratrol promoted odontogenic differentiation of DPSCs and up-regulated mRNA expression of RUNX2, DSPP, and DMP-1 in DPSCs; the expression of SIRT1 was the highest on the 7th day during odontogenic differentiation induction. Resveratrol resulted in the increasing protein expressions of SIRT1 and activated β-catenin when DPSCs was induced to odontogenic differentiation for 7 days.
Resveratrol promotes odontogenic differentiation of human DPSCs by up-regulating the expression of SIRT1 protein and activating β-catenin signaling pathway.
研究白藜芦醇是否通过上调沉默信息调节因子1（SIRT1）的表达并激活β-连环蛋白信号通路来促进人牙髓干细胞（DPSCs）的成牙分化。
用不同浓度的白藜芦醇（0、10、15、20和50 μmol/L）处理DPSCs 7天和14天，采用CCK-8检测细胞增殖活性。用15 μmol/L白藜芦醇诱导成牙分化7天后，进行碱性磷酸酶（ALP）染色，并用实时定量逆转录聚合酶链反应（qRT-PCR）检测DPSCs中与 runt 相关转录因子2（Runx2）、牙本质涎磷蛋白（DSPP）和牙本质基质蛋白-1（DMP-1）的mRNA表达。采用蛋白质免疫印迹法检测分化诱导后特定天数（0、第3、5、7和14天）DPSCs中SIRT1的表达。还用蛋白质免疫印迹法检测15 μmol/L白藜芦醇处理7天的DPSCs在成牙分化过程中SIRT1和激活的β-连环蛋白的表达。实验数据用GraphPad Prism 9软件包进行分析。
15 μmol/L白藜芦醇对第7天和第14天DPSCs的增殖无显著影响；15 μmol/L白藜芦醇促进DPSCs的成牙分化，并上调DPSCs中RUNX2、DSPP和DMP-1的mRNA表达；在成牙分化诱导过程中，SIRT1的表达在第7天最高。当DPSCs诱导成牙分化7天时，白藜芦醇导致SIRT1和激活的β-连环蛋白的蛋白表达增加。
白藜芦醇通过上调SIRT1蛋白的表达并激活β-连环蛋白信号通路促进人DPSCs的成牙分化。
