Faculty of Technology, University of Niš, Leskovac, Serbia.
Faculty of Medicine, University of Niš, Niš, Serbia.
Nat Prod Res. 2024 Sep;38(17):3080-3085. doi: 10.1080/14786419.2023.2208361. Epub 2023 May 8.
This work aimed to evaluate chemical profile, antioxidant activity and topical application safety of the raw Osage orange ( (Raf.) Schneid) fruit extracts obtained by maceration using ethanol and acetone. Out of eighteen different compounds registered in the extracts, fifteen were identified by ultra-high-performance liquid chromatography-tandem mass spectrometry. Pomiferin and osajin were characteristic and representative compounds in both ethanolic and acetone extracts of the Osage orange fruit. Both extracts showed good antioxidant activity (EC = 0.03 mg/cm) after 20 min of incubation. The topical administration safety of the extracts was evaluated by measuring skin biophysical parameters: electrical capacitance and erythema index, as indicators of hydration and irritation, respectively. Based on the results of the skin tests, it can be concluded that both of the Osage orange fruit extracts are safe for topical administration - they increased skin hydration and reduced skin irritation under the occlusion.
本研究旨在评估用乙醇和丙酮浸渍法从原始野樱桃（（Raf。）施耐德）果实提取物中获得的化学特征、抗氧化活性和局部应用安全性。在提取物中登记的十八种不同化合物中，有十五种通过超高效液相色谱-串联质谱法鉴定。在野樱桃果实的乙醇和丙酮提取物中，均含有特征性和代表性化合物Pomiferin 和 Osajin。两种提取物在孵育 20 分钟后均显示出良好的抗氧化活性（EC = 0.03 mg/cm）。通过测量皮肤生物物理参数：电容量和红斑指数，分别作为水合和刺激的指标，评估提取物的局部给药安全性。基于皮肤测试的结果，可以得出结论，两种野樱桃果实提取物均安全可用于局部给药 - 在封闭条件下，它们可增加皮肤水合度并减少皮肤刺激。
