Physical Examination Center, Handan Central Hospital, Congtai, Handan 056008, Hebei Province, China.
Department of Endocrinology, The First Medical Center, Chinese PLA General Hospital, Haidian, Beijing 100853, China.
Aging (Albany NY). 2023 May 5;15(9):3572-3585. doi: 10.18632/aging.204691.
Even today, thyroid cancer (THCA) remains an important threat to global health. For THCA patients, differentiated thyroid cancer is the most commonly identified pathological subtype, and those diagnosed with papillary thyroid cancer generally have good overall prognosis. For poorly differentiated subtype THCA, patients have aggressive disease course, higher risk of distant organ metastasis and inferior overall prognosis.
RNA-seq data from TCGA and GTEx databases are collected and analyzed via R. The correlation between SEMA6B expression level and pathological as well as clinical parameters of THCA patients was respectively investigated. Gene expression profiling and subsequent functional clustering analysis was the performed utilizing GSEA. The receiver operating characteristic (ROC) curve was utilized to evaluate the diagnostic value of SEMA6B expression.
Increased SEMA6B expression was characteristic in THCA tumor samples and was associated with specific pathologic and clinical features for TCHA patients. Univariate and multivariate analysis indicated that SEMA6B was independent predictive marker for THCA patients' prognosis. Gene expression profiling and functional clustering analysis suggested that SEMA6B high-expression was related with increased expression of multiple signal pathways and signatures of multiple immune cell infiltration.
In this study, through bioinformatic analysis and clinical data investigation, we demonstrated the potential value of SEMA6B as diagnostic and prognostic marker in THCA patient treatment.
即使在今天，甲状腺癌（THCA）仍然是全球健康的一个重要威胁。对于 THCA 患者，分化型甲状腺癌是最常见的病理亚型，而诊断为甲状腺乳头状癌的患者通常具有良好的总体预后。对于低分化型 THCA，患者的疾病具有侵袭性，远处器官转移的风险更高，总体预后较差。
通过 R 软件收集并分析来自 TCGA 和 GTEx 数据库的 RNA-seq 数据。分别研究了 SEMA6B 表达水平与 THCA 患者病理和临床参数之间的相关性。利用 GSEA 进行基因表达谱分析和随后的功能聚类分析。利用受试者工作特征（ROC）曲线评估 SEMA6B 表达的诊断价值。
THCA 肿瘤样本中 SEMA6B 的表达增加，并且与特定的 TCHA 患者病理和临床特征相关。单因素和多因素分析表明，SEMA6B 是 THCA 患者预后的独立预测标志物。基因表达谱分析和功能聚类分析表明，SEMA6B 高表达与多个信号通路的表达增加以及多种免疫细胞浸润的特征有关。
在这项研究中，通过生物信息学分析和临床数据调查，我们证明了 SEMA6B 作为 THCA 患者治疗中诊断和预后标志物的潜在价值。
