Jiang Yusha, Zhao Lang, Li Jia Danyang, Sun Jialiang, Miao Rui, Shao Bo, Wu Peifu
College of Life Sciences, Southwest Forestry University, Kunming, China.
J Adv Vet Anim Res. 2023 Mar 31;10(1):103-112. doi: 10.5455/javar.2023.j658. eCollection 2023 Mar.
This study aimed to pinpoint the universality of extracellular antimicrobial resistance elements (eAREs) and compare the contents of eAREs with those of intracellular AREs (iAREs) in animal feces, thus laying a foundation for the further analysis of the horizontal transfer of antimicrobial resistance genes (ARGs) in the animal guts.
Extracellular DNAs were isolated from the fecal samples of ( = 18), ( = 2), two breeds of broilers ( = 21 and 11, respectively), and from the contents of rabbit intestines ( = 5). eAREs were detected by PCR technology. iAREs in and broiler feces were also detected and compared with the corresponding eAREs. In addition, some gene cassettes of class 1 integrons were sequenced and analyzed.
The results showed that eAREs exist in animal feces and intestinal contents. In this study, different eAREs were detected from animal feces and intestinal contents, and , , , , class 1 integron, and IncFIB presented the highest detection rates. The detection rates of certain eAREs were significantly higher than those of parallel iAREs. The integral cassettes with intact structures were found in eAREs, and the cassettes carried ARGs.
The presented study here sheds light on the presence of eAREs in animal feces or guts, and eAREs may play an important role in the horizontal gene transfer of ARGs.
本研究旨在确定细胞外抗微生物抗性元件（eAREs）的普遍性，并比较动物粪便中eAREs与细胞内抗微生物抗性元件（iAREs）的含量，从而为进一步分析动物肠道中抗微生物抗性基因（ARGs）的水平转移奠定基础。
从18只猪、2只牛、两个品种的肉鸡（分别为21只和11只）的粪便样本以及5只兔子的肠道内容物中分离细胞外DNA。通过PCR技术检测eAREs。还检测了猪和肉鸡粪便中的iAREs，并与相应的eAREs进行比较。此外，对1类整合子的一些基因盒进行了测序和分析。
结果表明，eAREs存在于动物粪便和肠道内容物中。在本研究中，从动物粪便和肠道内容物中检测到不同的eAREs，tetM、tetQ、tetW、sul1、1类整合子和IncFIB的检出率最高。某些eAREs的检出率明显高于平行的iAREs。在eAREs中发现了结构完整的整合子盒，这些盒携带ARGs。
本研究揭示了动物粪便或肠道中存在eAREs，并且eAREs可能在ARGs的水平基因转移中发挥重要作用。
