National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Department of General Surgery, Shenzhen Children's Hospital, Shenzhen 518026, People's Republic of China.
Nanotechnology. 2023 May 24;34(32). doi: 10.1088/1361-6528/acd34e.
Inkjet printing, capable of rapid and template-free fabrication with high resolution and low material waste, is a promising method to construct electrochemical biosensor devices. However, the construction of fully inkjet-printed electrochemical biosensor remains a challenge owing to the lack of appropriate inks, especially the sensing inks of bioactive materials. Herein, we demonstrate a fully inkjet-printed, integrated and multiplexed electrochemical biosensor by combining rationally designed nanoparticle Inks. The stable gold (Au) nanoparticles ink with lower sintering temperature is prepared by using L-cysteine as stabilizer, and it is used to print the interconnects, the counter electrodes, and the working electrodes. The SU-8 ink is used to serve as dielectric layer for the biosensor, whereas the silver electrode is printed on the Au electrode by using commercially silver nanoparticles ink before it is chlorinated to prepare Ag/AgCl reference electrode. Moreover, we synthesize an inkjet-printable and electroactive ink, by the 'one-pot method', which is composed of conductive poly 6-aminoindole (PIn-6-NH) and gold-palladium (Au-Pd) alloy nanoparticle (Au-Pd@PIn-6-NH) to enhance the sensing performance of gold electrode towards hydrogen peroxide (HO). Especially, the amino groups in PIn-6-NHcan be further used to immobilizing glucose oxidase (GOx) and lactic acid oxidase (LOx) by glutaraldehyde to prepare printable sensing ink for the detection of glucose and lactate. The fully inkjet-printed electrochemical biosensor enabled by advanced inks can simultaneously detect glucose and lactate with good sensitivity and selectivity, as well as facile and scalable fabrication, showing great promise for metabolic monitoring.
喷墨打印具有快速、无需模板和高分辨率、低材料浪费的特点，是构建电化学生物传感器器件的一种很有前途的方法。然而，由于缺乏合适的墨水，尤其是生物活性材料的传感墨水，完全喷墨打印电化学生物传感器的构建仍然是一个挑战。在此，我们通过结合合理设计的纳米粒子墨水，展示了一种完全喷墨打印、集成和多路复用的电化学生物传感器。使用 L-半胱氨酸作为稳定剂，制备出具有较低烧结温度的稳定金（Au）纳米粒子墨水，并将其用于打印互连、对电极和工作电极。SU-8 墨水用作生物传感器的介电层，而商用银纳米粒子墨水则用于在 Au 电极上打印银电极，然后对其进行氯化以制备 Ag/AgCl 参比电极。此外，我们通过“一锅法”合成了一种可喷墨打印和电活性的墨水，由导电聚 6-氨基吲哚（PIn-6-NH）和金钯（Au-Pd）合金纳米粒子（Au-Pd@PIn-6-NH）组成，以增强金电极对过氧化氢（HO）的传感性能。特别是，PIn-6-NH 中的氨基可以进一步通过戊二醛固定葡萄糖氧化酶（GOx）和乳酸氧化酶（LOx），以制备用于检测葡萄糖和乳酸的可打印传感墨水。通过先进墨水实现的完全喷墨打印电化学生物传感器可以同时具有良好的灵敏度和选择性地检测葡萄糖和乳酸，并且具有易于制造和可扩展的特点，在代谢监测方面具有很大的应用前景。
