State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 210009, PR China.
Biosens Bioelectron. 2017 Jun 15;92:709-717. doi: 10.1016/j.bios.2016.10.013. Epub 2016 Oct 8.
The large-scale fabrication of nanocomposite based biosensors is always a challenge in the technology commercialization from laboratory to industry. In order to address this issue, we have designed a facile chemical method of fabricated nanocomposite ink applied to the screen-printed biosensor chip. This ink can be derived in the water through the in-situ growth of Prussian blue nanocubes (PBNCs) on the silver nanowires (AgNWs) to construct a composite nanostructure by a facile chemical method. Then a miniature flexible biosensor chip was screen-printed by using the prepared nanocomposite ink. Due to the synergic effects of the large specific surface area, high conductivity and electrocatalytic activity from AgNWs and PBNCs, the as-prepared biosensor chip exhibited a fast response (<3s)， a wider linear response from 0.01 to 1.3mM with an ultralow LOD=5µm, and the ultrahigh sensitivities of 131.31 and 481.20µAmMcm for the detections of glucose and hydrogen peroxide (HO), respectively. Furthermore, the biosensor chip exhibited excellent stability, good reproducibility and high anti-interference ability towards physiological substances under a very low working potential of -0.05. Hence, the proposed biosensor chip also showed a promising potential for the application in practical analysis.
基于纳米复合材料的生物传感器的大规模制造一直是从实验室到工业界实现技术商业化的一个挑战。为了解决这个问题，我们设计了一种简便的化学方法来制备纳米复合油墨，应用于丝网印刷生物传感器芯片。这种油墨可以通过普鲁士蓝纳米立方体（PBNCs）在银纳米线（AgNWs）上的原位生长，在水中得到，从而通过简便的化学方法构建复合纳米结构。然后，使用制备的纳米复合油墨对微型柔性生物传感器芯片进行丝网印刷。由于 AgNWs 和 PBNCs 的大比表面积、高导电性和电催化活性的协同效应，所制备的生物传感器芯片表现出快速响应（<3s），从 0.01 到 1.3mM 的线性响应范围更宽，检测葡萄糖和过氧化氢（HO）的超低压低检测限（LOD=5µm）分别为 5µm 和 131.31 和 481.20µAmMcm。此外，该生物传感器芯片在非常低的工作电位-0.05 下，对生理物质表现出优异的稳定性、良好的重现性和高抗干扰能力。因此，所提出的生物传感器芯片在实际分析中也显示出了很好的应用潜力。
