1 型、2 型糖尿病患者与健康个体的眼表微生物组比较分析。
Comparative analysis of the ocular surface microbiome in type-1, type-2 diabetes mellitus and healthy individuals.
机构信息
Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Ain-Helwan 11795, Cairo, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
出版信息
J Appl Microbiol. 2023 May 2;134(5). doi: 10.1093/jambio/lxad096.
AIM
Ocular health greatly impacts the quality of life, and diabetes mellitus (DM) is a major cause of several visual diseases. Likewise, microbiomes have a pivotal role in eye health. The aim was to study the effect of DM, both type-1 (T1DM) and type-2 (T2DM) on the ocular microbiome.
METHODS AND RESULTS
A total of 70 subjects were recruited for this study and divided into two main groups healthy nondiabetic (n = 18) and diabetic (28 T1DM and 24 T2DM). The ocular surface (OS) microbiome was more diverse in the healthy group than in the diabetic one. Taxonomic analysis revealed Proteobacteria as the main phylum (healthy nondiabetic 41.8%, T1DM 50.6%, and T2DM 52.5%), besides Streptococcus (healthy nondiabetic 16%, T1DM 26.75%, and T2DM 29.20%) and Paracoccus (healthy nondiabetic 17%, T1DM 34.85%, and T2DM 37.47%) as the main genera. No significant diversity was found between T1DM and T2DM on both phylum and genus levels; yet genera Brevundimonas and Leptotrichia were more significantly predominant in T1DM.
CONCLUSION
Two pathogenic genera, Streptococcus and Paracoccus, were more predominant in the DM group than in the healthy one.
目的
眼部健康极大地影响生活质量，糖尿病（DM）是几种视觉疾病的主要原因。同样，微生物组在眼部健康中起着关键作用。本研究旨在探讨 1 型（T1DM）和 2 型（T2DM）糖尿病对眼部微生物组的影响。
方法和结果
本研究共招募了 70 名受试者，分为两组：健康非糖尿病组（n=18）和糖尿病组（28 例 T1DM 和 24 例 T2DM）。健康组的眼表（OS）微生物组比糖尿病组更为多样化。分类分析显示，变形菌门（Proteobacteria）是主要的门（健康非糖尿病组 41.8%，T1DM 50.6%，T2DM 52.5%），此外还有链球菌属（Streptococcus）（健康非糖尿病组 16%，T1DM 26.75%，T2DM 29.20%）和副球菌属（Paracoccus）（健康非糖尿病组 17%，T1DM 34.85%，T2DM 37.47%）。在门和属水平上，T1DM 和 T2DM 之间没有发现显著的多样性；然而，Brevundimonas 和 Leptotrichia 这两个致病属在 T1DM 中更为显著。
结论
两种致病属链球菌属（Streptococcus）和副球菌属（Paracoccus）在糖尿病组中比在健康组中更为常见。
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