Zhu Xiangjia, Wei Ling, Rong Xianfang, Zhang Yinglei, Zhang Qian, Wen Xiaofeng, He Wenwen, Zhang Keke, Chen Feng, Wei Lai, Lu Yi
Department of Ophthalmology and Eye Institute, Ear, Nose and Throat (ENT) Hospital of Fudan University, Shanghai, China.
National Health Commission (NHC) Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Science, Shanghai, China.
Front Med (Lausanne). 2021 Apr 6;8:605639. doi: 10.3389/fmed.2021.605639. eCollection 2021.
Patients with type 2 diabetes mellitus (T2DM) are prone to ocular surface infections. We therefore characterized the conjunctival microbiome of T2DM patients and the influence of topical levofloxacin to investigate whether a dysbiosis is associated with this phenomenon. Conjunctival microbiome of 79 T2DM patients and 113 non-diabetic controls was profiled using the 16S rDNA sequencing approach. Furthermore, 21 T2DM and 14 non-diabetic patients who underwent cataract surgeries were followed up perioperatively and the influence of pre- and post-operative levofloxacin on the conjunctival microbiome was further investigated prospectively and compared longitudinally. The α-diversity of the conjunctival microbiota was significantly higher in T2DM patients than in controls ( < 0.05). Significant differences in both composition and function of the conjunctival microbiome were identified on the ocular surface of T2DM patients as compared to non-diabetic controls. Particularly, phylum and , genus , and were enriched, while genus was reduced on the T2DM ocular surface. Microbial genes functioning of bacterial chemotaxis was elevated in the conjunctival microbiome of T2DM patients. Furthermore, compared to the initial status, several genera including were more abundant in the conjunctival microbiome of T2DM patients after 3-days use of preoperative levofloxacin topically, while no genus was more abundant in the non-diabetic follow-up group. No difference was observed between initial status and 7 days after ceasing all postoperative medications in both diabetic and non-diabetic follow-up groups. The conjunctival microbiome of T2DM patients was more complex and may respond differently to topical antibiotics.
2型糖尿病（T2DM）患者易发生眼表感染。因此，我们对T2DM患者的结膜微生物群进行了特征分析，并研究了局部使用左氧氟沙星的影响，以调查生态失调是否与这一现象相关。采用16S rDNA测序方法对79例T2DM患者和113例非糖尿病对照者的结膜微生物群进行了分析。此外，对21例接受白内障手术的T2DM患者和14例非糖尿病患者进行围手术期随访，前瞻性地进一步研究术前和术后左氧氟沙星对结膜微生物群的影响，并进行纵向比较。T2DM患者结膜微生物群的α多样性显著高于对照组（<0.05）。与非糖尿病对照者相比，T2DM患者眼表结膜微生物群在组成和功能上均存在显著差异。特别是，在T2DM眼表，门 和 、属 和 富集，而属 减少。T2DM患者结膜微生物群中细菌趋化功能的微生物基因升高。此外，与初始状态相比，T2DM患者局部使用术前左氧氟沙星3天后，结膜微生物群中的几个属（包括 ）更为丰富，而非糖尿病随访组中没有属更为丰富。糖尿病和非糖尿病随访组在停止所有术后用药后初始状态与7天之间均未观察到差异。T2DM患者的结膜微生物群更为复杂，可能对局部抗生素有不同反应。
