Besrukow Paul, Will Frank, Dussling Stefan, Berkelmann-Löhnertz Beate, Schweiggert Ralf
Department of Beverage Research, Geisenheim University, Geisenheim, Germany.
Department of Crop Protection, Geisenheim University, Geisenheim, Germany.
Pest Manag Sci. 2023 Sep;79(9):3334-3341. doi: 10.1002/ps.7519. Epub 2023 May 30.
Organic viticulture seeks sustainable alternatives for eco-toxic copper fungicides to control downy mildew caused by Plasmopara viticola. (Poly)phenol-rich extracts of agricultural byproducts are known to possess antifungal activity, but high production costs often limit their actual implementation.
We developed and produced novel ligninsulfonate-based grape cane extract (GCE) formulations and an apple extract on a pilot plant scale, including a detailed (poly)phenol characterization by high-performance liquid chromatography photodiode array mass spectrometry (HPLC-PDA-MS). Our GCE formulations alone reduced downy mildew disease severity in greenhouse trials by 29%-69% in a dose-dependent manner, whereas a standard application of the copper-based agent alone reached ~56%. When applied together, disease severity was diminished by 78%-92%, revealing a synergistic effect that depended on the mixture ratio. Combining GCE formulations with the apple extract, additive effects were found (80% disease severity reduction).
The studied plant extracts are proposed to both substitute for and synergistically reinforce copper fungicides in grapevine downy mildew control. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
有机葡萄栽培寻求生态毒性铜杀菌剂的可持续替代品，以控制由葡萄生单轴霉引起的霜霉病。已知富含（多）酚的农业副产品提取物具有抗真菌活性，但高生产成本往往限制了它们的实际应用。
我们在中试规模上开发并生产了新型木质素磺酸盐基葡萄藤提取物（GCE）配方和苹果提取物，包括通过高效液相色谱光电二极管阵列质谱（HPLC-PDA-MS）对（多）酚进行详细表征。在温室试验中，仅我们的GCE配方就能以剂量依赖的方式将霜霉病严重程度降低29%-69%，而单独使用铜基药剂的标准施用量约为56%。当一起使用时，病害严重程度降低了78%-92%，显示出取决于混合比例的协同效应。将GCE配方与苹果提取物结合使用时，发现了相加效应（病害严重程度降低80%）。
所研究的植物提取物被提议在葡萄霜霉病防治中替代并协同增强铜杀菌剂。© 2023作者。《害虫管理科学》由约翰·威利父子有限公司代表化学工业协会出版。
