Firouzi Amandi Akram, Jokar Elham, Eslami Majid, Dadashpour Mehdi, Rezaie Mehdi, Yazdani Yalda, Nejati Babak
Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Semnan, Iran.
Department of Medical Chemistry, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Med Oncol. 2023 May 8;40(6):170. doi: 10.1007/s12032-023-02032-7.
Colorectal cancer (CRC) is the third broadly identified cancer in the world. The ineffectiveness of colorectal cancer treatment is redundantly reported. Natural bioactive compounds have gained popularity in reducing the drawback of conventional anti-cancer agents. Curcumin (Cur) and Artemisinin (Art) are materials of a natural source that have been utilized to treat numerous kinds of cancers. Although the benefits of bioactive materials, their utilization is limited because of poor solubility, bioavailability, and low dispersion rate in aqueous media. Nano delivery system such as niosome can improve the bioavailability and stability of bioactive compounds within the drug. In current work, we used Cur-Art co-loaded niosomal nanoparticles (Cur-Art NioNPs) as an anti-tumor factor versus colorectal cancer cell line. The synthesized formulations were characterized using dynamic light scattering, scanning electron microscopy, and FTIR. The proliferation ability of the cells and expression of apoptosis-associated gene were MTT assay and qRT-PCR, respectively. Cur-Art NioNPs exhibited well distributed with an encapsulation efficiency of 80.27% and 85.5% for Cur and Art. The NioNPs had good release and degradation properties, and had no negative effect on the survival and proliferation ability of SW480 cells. Importantly, nanoformulation form of Cur and Art significantly displayed higher toxicity effect against SW480 cells. Furthermore, Cur-Art NioNPs increased Bax, Fas, and p53 gene expressions and suppressed Bcl2, Rb, and Cyclin D 1 gene expressions. In summary, these results display the niosome NPs as a first report of nano-combinational application of the natural herbal substances with a one-step fabricated co-delivery system for effective colorectal cancer.
结直肠癌（CRC）是全球第三大常见癌症。结直肠癌治疗效果不佳的情况屡有报道。天然生物活性化合物在减少传统抗癌药物缺点方面受到欢迎。姜黄素（Cur）和青蒿素（Art）是已被用于治疗多种癌症的天然来源物质。尽管生物活性物质有诸多益处，但由于其在水性介质中的溶解度差、生物利用度低和分散速率低，其应用受到限制。纳米递送系统如囊泡可以提高生物活性化合物在药物中的生物利用度和稳定性。在当前工作中，我们使用姜黄素 - 青蒿素共负载囊泡纳米颗粒（Cur - Art NioNPs）作为针对结直肠癌细胞系 的抗肿瘤因子。使用动态光散射、扫描电子显微镜和傅里叶变换红外光谱对合成的制剂进行表征。细胞的增殖能力和凋亡相关基因的表达分别通过MTT 法和qRT - PCR检测。Cur - Art NioNPs分布均匀，Cur和Art的包封率分别为80.27%和85.5%。NioNPs具有良好的释放和降解特性，对SW480细胞的存活和增殖能力没有负面影响。重要的是，Cur和Art的纳米制剂形式对SW480细胞显示出更高的毒性作用。此外，Cur - Art NioNPs增加了Bax、Fas和p53基因的表达，并抑制了Bcl2、Rb和细胞周期蛋白D1基因的表达。总之，这些结果显示囊泡纳米颗粒是天然草药物质与一步制备的共递送系统用于有效治疗结直肠癌的纳米组合应用的首次报道。
