Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Curr Pharm Des. 2023;29(16):1300-1310. doi: 10.2174/1381612829666230508145533.
Osteosarcoma is a highly invasive and early metastatic tumor. At present, the toxic and side effects of chemotherapy affect the quality of life of cancer patients to varying degrees. Genipin is an extract of the natural medicine gardenia with various pharmacological activities.
The purpose of this study was to investigate the effect of Genipin on osteosarcoma and its potential mechanism of action.
Crystal violet staining, MTT assay and colony formation assay were used to detect the effect of genipin on the proliferation of osteosarcoma. The effects of vitexin on migration and invasion of osteosarcoma were detected by scratch healing assay and transwell assay. Hoechst staining and flow cytometry were used to detect the effect of genipin on apoptosis of osteosarcoma cells. The expression of related proteins was detected by Western blot. An orthotopic tumorigenic animal model was used to verify the effect of genipin on osteosarcoma in vivo.
The results of crystal violet staining, MTT method and colony formation method proved that genipin significantly inhibited the proliferation of osteosarcoma cells. The results of the scratch healing assay and transwell assay showed that gen significantly inhibited the migration and invasion of osteosarcoma cells. The results of Hoechst staining and flow cytometry showed that genipin significantly promoted the apoptosis of osteosarcoma cells. The results of animal experiments show that genipin has the same anti-tumor effect in vivo. Genipin may inhibit the growth of osteosarcoma through PI3K/AKT signaling.
Genipin can inhibit the growth of human osteosarcoma cells, and its mechanism may be related to the regulation of PI3K/AKT signaling pathway.
骨肉瘤是一种侵袭性强、早期转移的肿瘤。目前，化疗的毒性和副作用在不同程度上影响着癌症患者的生活质量。京尼平是一种天然药物栀子的提取物，具有多种药理活性。
本研究旨在探讨京尼平对骨肉瘤的作用及其潜在作用机制。
采用结晶紫染色、MTT 法和集落形成实验检测京尼平对骨肉瘤增殖的影响。划痕愈合实验和 Transwell 实验检测京尼平对骨肉瘤迁移和侵袭的影响。Hoechst 染色和流式细胞术检测京尼平对骨肉瘤细胞凋亡的影响。Western blot 检测相关蛋白的表达。建立骨肉瘤原位肿瘤动物模型，验证京尼平体内对骨肉瘤的作用。
结晶紫染色、MTT 法和集落形成实验结果表明，京尼平显著抑制骨肉瘤细胞的增殖。划痕愈合实验和 Transwell 实验结果表明，京尼平显著抑制骨肉瘤细胞的迁移和侵袭。Hoechst 染色和流式细胞术结果表明，京尼平显著促进骨肉瘤细胞的凋亡。动物实验结果表明，京尼平在体内也具有相同的抗肿瘤作用。京尼平可能通过 PI3K/AKT 信号通路抑制骨肉瘤的生长。
京尼平能抑制人骨肉瘤细胞的生长，其机制可能与调节 PI3K/AKT 信号通路有关。
