Spectroscopic atomic sample plane localization for precise digital holography.

In digital holography, the coherent scattered light fields can be reconstructed volumetrically. By refocusing the fields to the sample planes, absorption and phase-shift profiles of sparsely distributed samples can be simultaneously inferred in 3D. This holographic advantage is highly useful for spectroscopic imaging of cold atomic samples. However, unlike e.g. biological samples or solid particles, the quasi-thermal atomic gases under laser-cooling are typically featureless without sharp boundaries, invalidating a class of standard numerical refocusing methods. Here, we extend the refocusing protocol based on the Gouy phase anomaly for small phase objects to free atomic samples. With a prior knowledge on a coherent spectral phase angle relation for cold atoms that is robust against probe condition variations, an "out-of-phase" response of the atomic sample can be reliably identified, which flips the sign during the numeric back-propagation across the sample plane to serve as the refocus criterion. Experimentally, we determine the sample plane of a laser-cooled K gas released from a microscopic dipole trap, with a δz ≈ 1 µm ≪ 2λ/NA axial resolution, with a NA=0.3 holographic microscope at λ = 770 nm probe wavelength.