Owens M R, Cimino C D, Donnelly J
Thromb Res. 1986 May 1;42(3):343-54. doi: 10.1016/0049-3848(86)90263-x.
The effects of diethylstilbesterol (DES) on concentration of selected plasma coagulation proteins in male rats was studied sequentially over a 28-day period. At the outset of the study, male rats underwent orchiectomy and implantation of a pellet containing DES 5.0 mg or a placebo pellet. At intervals of 7 days, 3 of the animals from each treatment group were sacrificed and blood samples were withdrawn for assay. Plasma concentrations of Factor II (prothrombin) and Factor VII, two vitamin K-dependent coagulation proteins, were significantly decreased in DES-treated animals (approximately 70% normal activity for both Factors) compared to placebo-treated animals. In contrast, Factor VIII activity was higher with DES treatment than with placebo. Plasma concentrations of both antithrombin III (AT III) and plasminogen were decreased in DES-treated animals compared to placebo by immunologic as well as activity assays. Albumin concentrations were not different between the two groups at any point of study, although both were increased at day 21 compared to beginning values. Fibronectin concentrations were slightly decreased in DES-treated animals compared to those which received placebo. The combination of orchiectomy and treatment with DES had profound metabolic effects on the animals; the DES treated animals had a mean body weight loss of 100 g after 28 days, while the placebo group gained an average of 78 g compared to a control group (no surgery) which had average weight gain of 85 g during the 28 day period of study.
在28天的时间里，对己烯雌酚（DES）对雄性大鼠选定血浆凝血蛋白浓度的影响进行了连续研究。在研究开始时，雄性大鼠接受睾丸切除术，并植入含有5.0毫克DES的药丸或安慰剂药丸。每隔7天，从每个治疗组中处死3只动物，并采集血样进行检测。与安慰剂治疗的动物相比，DES治疗的动物中两种维生素K依赖性凝血蛋白——凝血因子II（凝血酶原）和凝血因子VII的血浆浓度显著降低（两种因子的活性约为正常活性的70%）。相比之下，DES治疗组的凝血因子VIII活性高于安慰剂组。通过免疫测定和活性测定，与安慰剂相比，DES治疗的动物中抗凝血酶III（AT III）和纤溶酶原的血浆浓度均降低。在研究的任何时间点，两组之间的白蛋白浓度均无差异，尽管与初始值相比，两组在第21天时白蛋白浓度均有所升高。与接受安慰剂的动物相比，DES治疗的动物中纤连蛋白浓度略有降低。睾丸切除术和DES治疗的联合对动物具有深远的代谢影响；DES治疗的动物在28天后平均体重减轻100克，而安慰剂组平均增重78克，相比之下，对照组（未手术）在28天的研究期间平均增重85克。
