Aesthet Surg J. 2023 Oct 13;43(11):NP924-NP933. doi: 10.1093/asj/sjad139.
Adipose-derived stem cells (ADSCs) are crucial in cell-assisted lipotransfer (CAL). ADSC-derived exosomes could improve the survival of CAL. Almost all relevant research now ignores ADSCs in favor of studying the proangiogenic potential of extracellular vesicles (EVs) on human umbilical vein endothelial cells (HUVECs).
Given the significance of ADSCs in CAL, the authors sought to verify that EVs from ADSCs under hypoxia treatment can enhance the angiogenic potential of ADSCs.
EVs were harvested from human ADSCs (hADSCs) under normoxia and hypoxia. A Cell Counting Kit-8 (CCK-8) assay was used to measure the proliferation of hADSCs. By examining the expression of CD31, vascular endothelial growth factor receptor 2, and vascular endothelial growth factor, the pro-angiogenic differentiation potential was assessed. Moreover, a tube formation experiment was carried out to evaluate the pro-angiogenic differentiation potential.
Hypoxic EVs showed more significant pro-proliferative and pro-angiogenic potential. Angiogenesis was more vigorous in hADSCs treated with hypoxic EVs than in those treated with nomorxic EVs. The hADSCs treated with hypoxic EVs expressed higher angiogenic markers, according to real-time polymerase chain reaction (RT-PCR) and Western blot analysis, which revealed more angiogenic marker expression in hypoxic EV-treated hADSCs. The same result was demonstrated by tube formation on Matrigel in vitro.
Hypoxic EVs significantly increased the proliferation and angiogenic differentiation potential of hADSCs. Hypoxic EV-treated ADSCs may be beneficial to CAL and prevascularized tissue-engineered constructs.
脂肪来源干细胞（ADSCs）在细胞辅助脂肪转移（CAL）中至关重要。ADSC 衍生的外泌体可以提高 CAL 的存活率。几乎所有相关研究现在都忽略了 ADSC，转而研究细胞外囊泡（EVs）对人脐静脉内皮细胞（HUVECs）的促血管生成潜力。
鉴于 ADSC 在 CAL 中的重要性，作者试图验证缺氧处理后的 ADSC 来源的 EV 可以增强 ADSC 的血管生成潜力。
从常氧和缺氧条件下的人 ADSC（hADSCs）中提取 EV。使用细胞计数试剂盒-8（CCK-8）检测 hADSCs 的增殖。通过检测 CD31、血管内皮生长因子受体 2 和血管内皮生长因子的表达，评估促血管生成分化潜能。此外，进行管形成实验来评估促血管生成分化潜能。
缺氧 EV 显示出更显著的促增殖和促血管生成潜力。与常氧 EV 处理的 hADSCs 相比，缺氧 EV 处理的 hADSCs 表现出更强的血管生成。根据实时聚合酶链反应（RT-PCR）和 Western blot 分析，hADSCs 用缺氧 EV 处理后表达更高的血管生成标志物，这表明缺氧 EV 处理的 hADSCs 表达更多的血管生成标志物。在体外 Matrigel 上的管形成实验也得到了同样的结果。
缺氧 EV 显著增加了 hADSCs 的增殖和血管生成分化潜力。缺氧 EV 处理的 ADSC 可能有益于 CAL 和预血管化组织工程构建体。
