Siegel L S, Johnson-Winegar A D, Sellin L C
Toxicol Appl Pharmacol. 1986 Jun 30;84(2):255-63. doi: 10.1016/0041-008x(86)90133-x.
To determine the efficacy of 3,4-diaminopyridine (3,4-DAP) as a potential treatment for botulism, its effect on the survival times of mice injected with type A, B, E, or F botulinum toxin (Bo Tx) was examined. Mice were injected ip with 10, 20, or 40 LD50 of Bo Tx. Three hours later, when the mice displayed signs of botulism, half of each group of mice was treated with 3,4-DAP, an agent which increases nerve-evoked transmitter release. At each dose of type A Bo Tx tested, 3,4-DAP definitely prolonged survival. In contrast, treatment with the drug did not significantly increase the survival time of mice injected with type B, E, or F Bo Tx. The differences in efficacy of 3,4-DAP against the four serotypes of Bo Tx together with previously reported variations in specific toxicity and duration of paralysis may reflect differences in the pharmacological activity of these neurotoxins.
为了确定3,4 - 二氨基吡啶（3,4 - DAP）作为肉毒中毒潜在治疗方法的疗效，研究了其对注射A、B、E或F型肉毒杆菌毒素（Bo Tx）小鼠存活时间的影响。给小鼠腹腔注射10、20或40个半数致死剂量（LD50）的Bo Tx。三小时后，当小鼠出现肉毒中毒症状时，每组小鼠的一半用3,4 - DAP治疗，3,4 - DAP是一种可增加神经诱发递质释放的药物。在测试的每种剂量的A型Bo Tx中，3,4 - DAP确实延长了存活时间。相比之下，该药物治疗并未显著增加注射B、E或F型Bo Tx小鼠的存活时间。3,4 - DAP对四种Bo Tx血清型疗效的差异以及先前报道的特定毒性和麻痹持续时间的变化可能反映了这些神经毒素药理活性的差异。
