Lu M H, Kennedy G L
Toxicol Appl Pharmacol. 1986 Jun 30;84(2):355-68. doi: 10.1016/0041-008x(86)90144-4.
Crl:CD rats were exposed (whole body) to mancozeb by inhalation at 0, 1, 17, 55, 110, 890, or 1890/500 mg/m3 for 6 hr/day from Day 6 through 15 of gestation (sperm-positive vaginal smear considered Day 1). Dams were killed 1 day prior to natural delivery and fetuses were examined externally, viscerally, and skeletally for any alterations. Maternal toxicity, as evidenced by significantly decreased body weight gain, hindlimb paralysis, general debilitation, and death or termination in extremis, was noted among rats exposed to mancozeb at concentrations of 500 to 1890 mg/m3. Dams from the 55 and 110 mg/m3 groups exhibited decreased body weight gain and hindlimb weakness. There was no maternal toxicity for dams exposed at a concentration of 17 mg/m3. Embryofetal toxicity, as evidenced by a significantly increased incidence of totally resorbed litters, external hemorrhage, and wavy ribs, was noted at concentrations of 55 mg/m3 and above. The embryofetal toxicity occurred only at concentrations toxic to the dam. Among the groups exposed to mancozeb, the incidence of major malformations was not dose related. Hence, under the test conditions of this study, mancozeb was not found to be teratogenic and produced no toxicity unique to the conceptus.
将妊娠第6天至第15天（以精子阳性阴道涂片视为第1天）的Crl:CD大鼠全身暴露于代森锰锌中，吸入浓度分别为0、1、17、55、110、890或1890/500mg/m³，每天暴露6小时。在自然分娩前1天处死母鼠，并对胎儿进行外部、内脏和骨骼检查，以观察有无异常。在暴露于浓度为500至1890mg/m³代森锰锌的大鼠中，观察到母体毒性，表现为体重增加显著减少、后肢麻痹、全身虚弱以及死亡或濒死期终止。55和110mg/m³组的母鼠体重增加减少且后肢无力。暴露于浓度为17mg/m³的母鼠未出现母体毒性。在浓度为55mg/m³及以上时，观察到胚胎-胎儿毒性，表现为完全吸收窝仔数、外部出血和肋骨波浪状的发生率显著增加。胚胎-胎儿毒性仅在对母鼠有毒的浓度下出现。在暴露于代森锰锌的组中，主要畸形的发生率与剂量无关。因此，在本研究的试验条件下，未发现代森锰锌具有致畸性，也未产生对胚胎独特的毒性。
