Hayes E P, Scala P, Witz G
Toxicol Lett. 1986 May;31(2):139-45. doi: 10.1016/0378-4274(86)90007-x.
The metabolism of certain compounds by rat hepatic microsomal preparations obtained from acetone-treated rats is greater than that measured in microsomes obtained from control rats. Since the lipid composition of membranes can significantly influence metabolism catalyzed by membrane-bound enzymes, studies were conducted in order to determine whether acetone-induced alterations in microsomal metabolism are associated with changes in the lipid composition of these membranes. Phospholipid and cholesterol concentrations were measured in extracts of rat liver microsomes obtained from rats treated with acetone, sodium phenobarbital, or vehicle. Acetone treatment did not alter the phospholipid and cholesterol content of the microsomes, whereas sodium phenobarbital (positive control) caused a significant decrease in cholesterol content/mg microsomal protein. These data indicate that acetone-induced alterations in microsomal metabolism may not be attributed to changes in the phospholipid and cholesterol content of the microsomes.
从用丙酮处理过的大鼠获得的肝微粒体制剂对某些化合物的代谢作用，比从对照大鼠获得的微粒体中所测得的代谢作用更强。由于膜的脂质组成可显著影响膜结合酶催化的代谢过程，因此开展了研究以确定丙酮诱导的微粒体代谢改变是否与这些膜的脂质组成变化相关。测定了从用丙酮、苯巴比妥钠或赋形剂处理过的大鼠获得的肝微粒体提取物中的磷脂和胆固醇浓度。丙酮处理未改变微粒体的磷脂和胆固醇含量，而苯巴比妥钠（阳性对照）使每毫克微粒体蛋白中的胆固醇含量显著降低。这些数据表明，丙酮诱导的微粒体代谢改变可能并非归因于微粒体磷脂和胆固醇含量的变化。
